High-fidelity imaging with bessel-beam light-sheet microscopy for whole-brain structural and functional studies
Authors: Mu¨llenbroich M.C., Silvestri L., Turrini L., Di Giovanna A.P., Alterini T., Gheisari A., Sacconi L., Vanzi F., Pavone F.S.
Autors Affiliation: European Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Via Nello Carrara, 1, Sesto Fiorentino (FI), 50019, Italy; National Institute of Optics, National Research Council, Via Nello Carrara, 1, Sesto Fiorentino (FI), 50019, Italy; Department of Physics and Astronomy, University of Florence, Via G. Sansone, 1, Sesto Fiorentino (FI), 50019, Italy
Abstract: Bessel beam have attractive properties like their propagation invariance and “selfhealing“ capabilities. Here we present significant advantages of Bessel illumination in structural and functional imaging in light-sheet microscopy compared to conventional Gaussian beam illumination.
KeyWords: Bessel functions; Laser beams; Light, Bessel beam; Functional imaging; Gaussian beam illumination; High-fidelity; Light-sheet microscopies; Propagation invariance; Self-healing; Whole brains, Gaussian beamsDOI: 10.1364/BRAIN.2017.BrW4B.2