Towards excimer-laser-based stereolithography: A rapid process to fabricate rigid biodegradable photopolymer scaffolds
Authors: Beke S., Anjum F., Tsushima H., Ceseracciu L., Chieregatti E., Diaspro A., Athanassiou A., Brandi F.
Autors Affiliation: Department of Nanophysics, Istituto Italiano di Tecnologia (IIT), 16152 Genova, Italy; Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia (IIT), via Morego 30, 16152 Genova, Italy; Department of Physics, University of Genoa, via Balbi 5, 16126 Genova, Italy; Center for Biomolecular Nanotechnologies at UNILE, Istituto Italiano di Tecnologia, Via Barsanti, 73010 Arnesano (LE), Italy; National Nanotechnology Laboratory (NNL), CNR – Istituto di Nanoscienze, via per Arnesano, 73100 Lecce, Italy
Abstract: We demonstrate high-resolution photocross-linking of biodegradable poly(propylene fumarate) (PPF) and diethyl fumarate (DEF) using UV excimer laser photocuring at 308 nm. The curing depth can be tuned in a micrometre range by adjusting the total energy dose (total fluence). Young\’s moduli of the scaffolds are found to be a few gigapascal, high enough to support bone formation. The results presented here demonstrate that the proposed technique is an excellent tool for the fabrication of stiff and biocompatible structures on a micrometre scale with defined patterns of high resolution in all three spatial dimensions. Using UV laser photocuring at 308 nm will significantly improve the speed of rapid prototyping of biocompatible and biodegradable polymer scaffolds and enables its production in a few seconds, providing high lateral and horizontal resolution. This short timescale is indeed a tremendous asset that will enable a more efficient translation of technology to clinical applications. Preliminary cell tests proved that PPF : DEF scaffolds produced by excimer laser photocuring are biocompatible and, therefore, are promising candidates to be applied in tissue engineering and regenerative medicine.
Journal/Review: JOURNAL OF THE ROYAL SOCIETY INTERFACE
Volume: 9 (76) Pages from: 3017 to: 3026
KeyWords: Laser photocuring; Scaffolds; Tissue engineering; Biocompatibility; Poly(propylene fumarate)DOI: 10.1098/rsif.2012.0300Citations: 28data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2019-11-10References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here