Laser-assisted synthesis of Staphylococcus aureus protein-capped silicon quantum dots as bio-functional nanoprobes
Authors: Bagga K., Barchanski A., Intartaglia R., Dante S., Marotta R., Diaspro A., Sajti C.L., Brandi F.
Autors Affiliation: Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, Italy; Laser Zentrum Hannover E.V., Nanotechnology Department, Hollerithallee 8, D-30419 Hannover, Germany; Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, Italy; Nanochemistry, Istituto Italiano di Tecnologia, via Morego, 30, I-16163 Genova, Italy
Abstract: A novel approach for nanofabricating protein-functionalized luminescent silicon nanoparticles based on infrared ultrafast laser ablation of silicon in an aqueous solution of Staphylococcus aureus protein A is reported. It is demonstrated that 8 nm protein A-capped silicon quantum dots with blue-green photoemissive properties are generated. The conjugation efficiency studies reveal a high percentage of protein A attached to the Si nanoparticle surface through physical adsorption phenomena during the in situ laser process. The biological functionality of laser-generated Staphylococcus aureus protein A-capped Si nanoparticles is investigated. Confocal and electron microscopy together with energy dispersive x-ray spectroscopy analysis show that these Si-based bio-nanostructures selectively bind IgG in the cells. Cell viability studies reveal that these protein A-capped Si nanoparticles are suitable for biological applications, demonstrating their potential as universal secondary biomarkers for in vivo applications such as long-term, real-time cell labeling, cell staining and controlled drug delivery.
Journal/Review: LASER PHYSICS LETTERS
Volume: 10 (6) Pages from: 065603-1 to: 065603-8
More Information: The authors gratefully acknowledge Dr Alberto Curcio for helping with the confocal microscopy experiments. AB and CLS thank the German Research Foundation within the Excellence Cluster REBIRTH for funding.KeyWords: NANOPARTICLES; CELLS; PHOTOSENSITIZERS; CYTOTOXICITY; SURFACESDOI: 10.1088/1612-2011/10/6/065603