Brain imaging from the nano- to the macro-scale
Authors: Silvestri L., Allegra Mascaro A.L., Costantini I., Sacconi L., Pavone F.S.
Autors Affiliation: National Institute of Optics (INO-CNR), Sesto Fiorentino, 50019, Italy; European Laboratory for Non-linear Spectroscopy, Sesto Fiorentino, 50019, Italy
Abstract: A single imaging technique can reveal only a small part of the brains\’ complex machinery. Here, we present a correlative framework to combine complementary imaging technique and obtain a more comprehensive view of the encephalon. The same neuron imaged in vivo with two-photon fluorescence microscopy could be retrieved ex-vivo and characterized in terms of its ultrastructural features by means of electron microscopy. On the other hand, we describe a method to integrate data from in vivo two-photon fluorescence imaging and ex vivo light sheet microscopy, based on the use of major blood vessels as reference chart. This correlative approach allows placing in a three-dimensional anatomic context the neurons whose dynamics have been observed with high detail in vivo.
KeyWords: Brain mapping; Fluorescence; Fluorescence microscopy; Imaging techniques; Machinery; Neuroimaging; Photons, Brain imaging; Complex machinery; Correlative approaches; Ex-vivo; Light-sheet microscopies; Macro scale; Two-photon fluorescence imaging; Two-photon fluorescence microscopy, Blood vesselsDOI: 10.1109/NER.2015.7146760