Whole brain optical imaging
Authors: Silvestri L., Allegra Mascaro A.L., Costantini I., Sacconi L., Pavone F.S.
Autors Affiliation: National Institute of Optics (INO-CNR), Via Nello Carrara, 1, Sesto Fiorentino (FI), 50019, Italy; European Laboratory for Non-linear Spectroscopy (LENS), Via Nello Carrara, 1, Sesto Fiorentino (FI), 50019, Italy; Department of Physics and Astronomy, University of Florence, Via G. Sansone, 1, Sesto Fiorentino (FI), 50019, Italy; International Center for Computational Neurophotonics (ICON Foundation), Via Nello Carrara, 1, Sesto Fiorentino (FI), 50019, Italy
Abstract: Nowadays, there are several imaging techniques offering a complementary approach to visualize intact neural networks on large areas. Each of those offers a different strategy and furnish complementary information on the role of neural components. We will describe different approaches enabling to move from single neuron details to whole brain imaging, connecting short range structural information to long range one. In particular, some examples of correlative microscopies, combining linear and non linear techniques will also be described.
More Information: Sponsors: Ente Cassa di Risparmio di Firenze. European Commission, EC. – Plexon Inc.; The Society of Photo-Optical Instrumentation Engineers (SPIE)KeyWords: Brain mapping; Imaging techniques; Neuroimaging, Correlative microscopies; Multi-modal imaging; neurophotonics; Nonlinear techniques; Optical imaging; Single neuron; Structural imaging; Structural information, NeurosurgeryDOI: 10.1117/12.2087339