Scientific Results

Optimal staining and clearing protocol for whole mouse brain vasculature imaging with light-sheet microscopy

Year: 2017

Authors: Di Giovanna A.P., Tibo A., Silvestri L., Muellenbroich M.C., Costantini I., Sacconi L., Frasconi P., Pavone F.S.

Autors Affiliation: European Laboratory for Non-linear Spectroscopy, University of Florence, Italy; Department of Information Engineering (DINFO), University of Florence, Italy; National Institute of Optics, National Research Council, Italy; Department of Physics and Astronomy, University of Florence, Italy

Abstract: Light-sheet microscopy enables whole mouse brain imaging in association with clearing methodologies. Here, we present a pipeline for optimal investigation of the vascular component, which offers improved image quality for morphological analysis.

Conference title:

KeyWords: Brain mapping; Image enhancement; Microscopic examination; Neuroimaging, Light-sheet microscopies; Morphological analysis; Mouse brain, Mammals

This site uses cookies. If you decide to continue browsing we consider that you accept their use. For more information about cookies and how to delete them please read our Info Policy on cookies use.
Read more