Optimal staining and clearing protocol for whole mouse brain vasculature imaging with light-sheet microscopy
Authors: Di Giovanna A.P., Tibo A., Silvestri L., Muellenbroich M.C., Costantini I., Sacconi L., Frasconi P., Pavone F.S.
Autors Affiliation: European Laboratory for Non-linear Spectroscopy, University of Florence, Italy; Department of Information Engineering (DINFO), University of Florence, Italy; National Institute of Optics, National Research Council, Italy; Department of Physics and Astronomy, University of Florence, Italy
Abstract: Light-sheet microscopy enables whole mouse brain imaging in association with clearing methodologies. Here, we present a pipeline for optimal investigation of the vascular component, which offers improved image quality for morphological analysis.
KeyWords: Brain mapping; Image enhancement; Microscopic examination; Neuroimaging, Light-sheet microscopies; Morphological analysis; Mouse brain, Mammals