Scientific Results

Fast, image-based autofocus system for high-resolution optical microscopy of whole mouse brains

Year: 2017

Authors: Silvestri L., M├╝llenbroich M.C., Costantini I., Di Giovanna A.P., Sacconi L., Pavone F.S.

Autors Affiliation: National Institute of Optics, National Research Council (INO-CNR), Via Nello Carrara, 1, Sesto Fiorentino (FI), 50019, Italy; European Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Via Nello Carrara, 1, Sesto Fiorentino (FI), 50019, Italy; Department of Physics and Astronomy, University of Florence, Via G. Sansone, 1, Sesto Fiorentino (FI), 50019, Italy

Abstract: We describe an image-based method for single-shot measurement and real-time correction of defocus in an optical microscope. The method is validated both in bright-field, epifluorescence and light-sheet microscopy.

Conference title:

KeyWords: Auto-focus system; Bright fields; Epifluorescences; High-resolution optical microscopy; Image-based methods; Light-sheet microscopies; Real-time corrections; Single-shot measurements
DOI: 10.1364/BODA.2017.JTu4A.8

This site uses cookies. If you decide to continue browsing we consider that you accept their use. For more information about cookies and how to delete them please read our Info Policy on cookies use.
Read more