Fast, image-based autofocus system for high-resolution optical microscopy of whole mouse brains
Authors: Silvestri L., Müllenbroich M.C., Costantini I., Di Giovanna A.P., Sacconi L., Pavone F.S.
Autors Affiliation: National Institute of Optics, National Research Council (INO-CNR), Via Nello Carrara, 1, Sesto Fiorentino (FI), 50019, Italy; European Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Via Nello Carrara, 1, Sesto Fiorentino (FI), 50019, Italy; Department of Physics and Astronomy, University of Florence, Via G. Sansone, 1, Sesto Fiorentino (FI), 50019, Italy
Abstract: We describe an image-based method for single-shot measurement and real-time correction of defocus in an optical microscope. The method is validated both in bright-field, epifluorescence and light-sheet microscopy.
KeyWords: Auto-focus system; Bright fields; Epifluorescences; High-resolution optical microscopy; Image-based methods; Light-sheet microscopies; Real-time corrections; Single-shot measurementsDOI: 10.1364/BODA.2017.JTu4A.8