Correlative polarized light imaging and two-photon fluorescence microscopy for 3D myelinated fibers reconstruction

Year: 2017

Authors: Costantini I., Menzel M., Silvestri L., Schubert N., Axer M., Amunts K., Pavone F.S.

Autors Affiliation: European Laboratory for Non-linear Spectroscopy, University of Florence, Italy; Institute of Neuroscience and Medicine (INM-1), Research Centre Jülich, Jülich, Germany; C. and O. Vogt Institute of Brain Research, Heinrich Heine University Düsseldorf, Düsseldorf, Germany; National Institute of Optics, National Research Council, Italy; Department of Physics, University of Florence, Italy

Abstract: In this work, we employ an integrated label-free dual approach that combines Polarized Light Imaging with Two-Photon Fluorescence Microscopy to study fiber orientations of myelinated axons in fixed brain slices from different species. © 2017 SPIE-OSA.

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KeyWords: Fluorescence; Fluorescence microscopy; Image reconstruction; Light polarization; Photons, Brain slices; Dual approach; Label free; Myelinated axons; Myelinated fibers; Polarized light imaging; Two-photon fluorescence microscopy, Light