Holografic microscope able to generate an image with extended focus without mechanical movement
Authors: Ferraro P., Grilli S., De Natale P., Alfieri D., Pierattini G., Finizio A., De Nicola S., Coppola G., Striano V.
Autors Affiliation: Istituto Nazionale di Ottica Applicata, Sez. di Napoli, Via Campi Flegrei 34, Pozzuoli (NA), 80078, Italy; Istituto di Cibernetica Del CNR, Via Campi Flegrei 34, Pozzuoli (NA), 80078, Italy; Istituto di Microelettronica e Microsistemi Del CNR, Via P. Castellini 111, Napoli, 80100, Italy
Abstract: In microscopy high magnifications are achievable for investigating micro-objects but the paradigm is that higher is the required magnification lower the depth of focus is. For an object having a 3D complex shape only a portion of it appears in well focus to the observer that essentially is looking at a single image plane. If an accurate analysis of the whole object has to be performed it is necessary to have a single sharp image in which all details of the object. We propose an holographic microscope to construct an EFI without any mechanical scanning or special optical components. We demonstrate that by this novel approach using DH it is possible to obtain EFI of a 3D object without any mechanical scanning. It will be shown that the unique property in DH, that the phase information of the reconstructed wave front is available numerically allows to construct an EFI image of the object without mechanical movement and by a single image. The proposed microscope could be useful to conduct investigation for experiments in microgravity conditions.
KeyWords: e da rivista: Kinematics; Mechanics; Scanning; Wavefronts, Accurate analysis; Depth of focus; High magnifications; Mechanical movements; Mechanical scanning; Microgravity conditions; Optical components; Phase information, Microscopes