Blue light-irradiated human keloid fibroblasts: an in vitro study
Authors: Magni G., Rossi F., Tatini F., Pini R., Coppi E., Cherchi F., Fusco I., Pugliese AM., Pedata F., Fraccalvieri M., Gasperini, S Pavone FS., Tripodi C., Alfieri D., Targetti L.
Autors Affiliation: [Magni, Giada; Rossi, Francesca; Tatini, Francesca; Pini, Roberto] CNR, Ist Fis Applicata, Via Madonna del Piano 10, I-50019 Sesto Fiorentino, FI, Italy.
[Coppi, Elisabetta; Cherchi, Federica; Fusco, Irene; Pugliese, Anna Maria; Pedata, Felicita] Univ Florence, Dept NEUROFARBA, Div Pharmacol & Toxicol, Viale Pieraccini 6, I-50139 Florence, Italy.
[Fraccalvieri, Marco] Osped San Lazzaro, AOU Citta Salute & Sci Torino, Corso Bramante 88, I-10126 Turin, Italy.
[Gasperini, Stefano] Med Advisor SaS, Via Cavour 4, I-56127 Pisa, Italy.
[Pavone, Francesco S.] CNR, Natl Inst Opt, Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy.
[Pavone, Francesco S.] European Lab Nonlinear Spect LENS, Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy.
[Pavone, Francesco S.] Univ Florence, Dept Phys, Via G Sansone 1, I-50019 Sesto Fiorentino, Italy.
[Tripodi, Cristina; Alfieri, Domenico; Targetti, Lorenzo] EmoLed Srl, Via Rimaggio 141, I-50019 Sesto Fiorentino, FI, Italy.
Abstract: Blue LED light irradiation is currently under investigation because of its effect in wound healing improvement. In this context, several mechanisms of action are likely to occur at the same time, consistently with the presence of different light absorbers within the skin. In our previous studies we observed the wound healing in superficial abrasions in an in vivo murine model. The results evidenced that both inflammatory infiltrate and myofibroblasts activity increase after irradiation. In this study we focused on evaluating the consequences of light absorption in fibroblasts from human cells culture: they play a key role in wound healing, both in physiological conditions and in pathological ones, such as keloid scarring. In particular we used keloids fibroblasts as a new target in order to investigate a possible metabolic or cellular mechanism correlation. Human keloid tissues were excised during standard surgery and immediately underwent primary cell culture extraction. Fibroblasts were allowed to grow in the appropriate conditions and then exposed to blue light. A metabolic colorimetric test (WST-8) was then performed. The tests evidenced an effect in mitochondrial activity, which could be modulated by the duration of the treatment. Electrophysiology pointed out a different behavior of irradiated fibroblasts. In conclusion, the Blue LED light affects the metabolic activity of fibroblasts and thus the cellular proliferation rate. No specific effect was found on keloid fibroblasts, thus indicating a very basic intracellular component, such as cytochromes, being the target of the treatment. WOS:000446253900004
Conference title: Conference on Mechanisms of Photobiomodulation Therapy XIII
Place: San Francisco, CA JAN 27-28, 2018
KeyWords: Wound Healing; Blue LED light; human keloid fibroblast; Cells; pathomechanisms