Tumour detection and staging through multimodal fibre-probe spectroscopy
Authors: Baria E., Anand S., Fantechi R., Morselli S., Gacci M., Carini M., Cicchi R., Pavone FS.
Autors Affiliation: Natl Inst Opt, Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy; Univ Florence, Dept Clin & Expt Med, Div Urol, Largo Brambilla 3, I-50134 Florence, Italy; Univ Florence, European Lab Nonlinear Spect, Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy; Univ Florence, Dept Phys, Via Sansone 1, I-50019 Sesto Fiorentino, Italy
Abstract: Urothelial carcinoma (UC) is the most common type of bladder cancer. The gold standard for detecting UC is white-light cystoscopy, which is followed by tissue biopsy and pathological examination. However, such process is invasive, timeconsuming and prone to sampling errors. In this framework, optical spectroscopy techniques provide fast, label-free and non-invasive alternatives to standard histopathology. Thus, the aim of this study is to discriminate normal bladder tissues from urothelial tumours, and to identify the different stages of the disease, by means of combined auto-fluorescence, diffuse reflectance and Raman spectroscopy. In fact, these techniques were implemented in a compact and transportable setup based on two optical fibre probes: one coupled to fluorescence and reflectance excitation sources, while the other one to the 785 nm laser. Raman, fluorescence and reflected light signals were collected though the same probe used for excitation and sent to a spectrograph. We used this experimental setup for studying fresh biopsies of urothelial tumour and healthy bladder collected from 32 patients undergoing Transurethral Resection of Bladder Tumours (TURBT). Scoring methods based on ratiometric approach and Principal Component Analysis (PCA) allowed not only to discriminate healthy biopsies from tumour ones, but also to recognize three tumour stages.
Conference title: Conference on Biophotonics – Photonic Solutions for Better Health Care VI
KeyWords: Biopsy; Fluorescence; Health care; Laser excitation; Optical fibers; Photonics; Probes; Reflection; Spectroscopy; Tumors; Uranium compounds, bladder; Diffuse reflectance; Excitation sources; Optical spectroscopy techniques; Raman; Transurethral resections; Two-optical fibre probe; Urothelial carcinoma, Principal component analysisDOI: 10.1117/12.2317996