Scientific Results

Surface-plasmon optical-heterodyne clock biosensor

Year: 2018

Authors: Giorgini A., Avino S., Malara P., Zullo R., De Natale P., Mrkvová K., Homola J., Gagliardi G.

Autors Affiliation: Consiglio Nazionale delle Ricerche, Istituto Nazionale di Ottica (INO), via Campi Flegrei 34, 80078 Pozzuoli (Naples), Italy; Consiglio Nazionale delle Ricerche, Istituto Nazionale di Ottica (INO), Largo E. Fermi 6, 50125 Firenze, Italy; Institute of Photonics and Electronics, Czech Academy of Sciences, Chaberská 57, 18251 Prague, Czech Republic

Abstract: Surface-plasmon resonance (SPR) sensors represent a formidable technology for molecular biology and bioanalytics applications. Here, we devise a new interrogation architecture that transforms a standard SPR chip into an optical-heterodyne clock detector comparable with the best SPR instruments. The key ingredients are (i) the conversion of refractive-index changes on the SPR chip into frequency shifts of an oscillatory electronic signal (ii) a differential probe & reference optical-cavity scheme that cancels out most source/detector noise contributions. A rigorous characterization of the sensor performance with the integration time demonstrates a refractive-index resolution on the 10−8-RIU level over a 1-s timescale using a bare, unstabilized SPR element. A bio-sensing test with a functionalized surface interfaced with a microfluidic flow-cell is carried out, showing detection of streptavidin via covalent bond down to concentrations of 90 fg/mm2.

Journal/Review: SENSORS AND ACTUATORS. B, CHEMICAL (PRINT)

Volume: 273      Pages from: 336  to: 341

More Information: Funding sponsor: College of Natural Resources, University of California Berkeley, CNR. Grantová Agentura Ceské Republiky, GACR, GBP205/12/G118. Ministero dell’Istruzione, dell’Università e della Ricerca, MIUR, PON01_01525. – The experimental work was partially supported by Italian Ministry for Education and Research (MIUR) with project “MONICA” (PON01_01525), by the P.O.R. Campania FESR 2007-2013 project titled \”BERSAGLI” and by the Czech Science Foundation under contract (# GBP205/12/G118). AG thanks CNR “Short Term Mobility” program that supported his visit at IPE, Prague. J . H. acknowledges support by the Praemium Academiae of the Czech Academy of Sciences. The authors are indebted with Luciano De Petrocellis (CNR-ICB) for his precious advices. The authors thank G. Notariale (CNR-INO) for assistance on the electronic equipment. Appendix A
KeyWords: Clocks; Heterodyne detection; Molecular biology; Optical frequency conversion; Optical resonators; Plasmons; Proteins; Refractive index; Surface plasmon resonance, Biosensing; Differential probes; Electronic signals; Functionalized surfaces; Microfluidic flow cell; Noise contributions; Refractive index changes; Surface plasmon resonance sensor, Optical heterodyning
DOI: 10.1016/j.snb.2018.06.028

Citations: 8
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