Scientific Results

Surface-plasmon optical-heterodyne clock biosensor

Year: 2018

Authors: Giorgini A., Avino S., Malara P., Zullo R., De Natale P., Mrkvová K., Homola J., Gagliardi G.

Autors Affiliation: Consiglio Nazionale delle Ricerche, Istituto Nazionale di Ottica (INO), via Campi Flegrei 34, 80078 Pozzuoli (Naples), Italy; Consiglio Nazionale delle Ricerche, Istituto Nazionale di Ottica (INO), Largo E. Fermi 6, 50125 Firenze, Italy; Institute of Photonics and Electronics, Czech Academy of Sciences, Chaberská 57, 18251 Prague, Czech Republic

Abstract: Surface-plasmon resonance (SPR) sensors represent a formidable technology for molecular biology and bioanalytics applications. Here, we devise a new interrogation architecture that transforms a standard SPR chip into an optical-heterodyne clock detector comparable with the best SPR instruments. The key ingredients are (i) the conversion of refractive-index changes on the SPR chip into frequency shifts of an oscillatory electronic signal (ii) a differential probe & reference optical-cavity scheme that cancels out most source/detector noise contributions. A rigorous characterization of the sensor performance with the integration time demonstrates a refractive-index resolution on the 10−8-RIU level over a 1-s timescale using a bare, unstabilized SPR element. A bio-sensing test with a functionalized surface interfaced with a microfluidic flow-cell is carried out, showing detection of streptavidin via covalent bond down to concentrations of 90 fg/mm2.


Volume: 273      Pages from: 336  to: 341

More Information: Funding sponsor: College of Natural Resources, University of California Berkeley, CNR. Grantová Agentura Ceské Republiky, GACR, GBP205/12/G118. Ministero dell’Istruzione, dell’Università e della Ricerca, MIUR, PON01_01525. – The experimental work was partially supported by Italian Ministry for Education and Research (MIUR) with project “MONICA” (PON01_01525), by the P.O.R. Campania FESR 2007-2013 project titled \”BERSAGLI” and by the Czech Science Foundation under contract (# GBP205/12/G118). AG thanks CNR “Short Term Mobility” program that supported his visit at IPE, Prague. J . H. acknowledges support by the Praemium Academiae of the Czech Academy of Sciences. The authors are indebted with Luciano De Petrocellis (CNR-ICB) for his precious advices. The authors thank G. Notariale (CNR-INO) for assistance on the electronic equipment. Appendix A
KeyWords: Clocks; Heterodyne detection; Molecular biology; Optical frequency conversion; Optical resonators; Plasmons; Proteins; Refractive index; Surface plasmon resonance, Biosensing; Differential probes; Electronic signals; Functionalized surfaces; Microfluidic flow cell; Noise contributions; Refractive index changes; Surface plasmon resonance sensor, Optical heterodyning
DOI: 10.1016/j.snb.2018.06.028

Citations: 9
data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2022-01-16
References taken from IsiWeb of Knowledge: (subscribers only)
Connecting to view paper tab on IsiWeb: Click here
Connecting to view citations from IsiWeb: Click here

This site uses cookies. If you decide to continue browsing we consider that you accept their use. For more information about cookies and how to delete them please read our Info Policy on cookies use.
Read more