Liquid Crystalline Networks toward Regenerative Medicine and Tissue Repair
Authors: Martella D., Paoli P., Pioner J.M., Sacconi L., Coppini R., Santini L., Lulli M., Cerbai E., Wiersma D.S., Poggesi C., Ferrantini C., Parmeggiani C.
Autors Affiliation: European Laboratory for Non-Linear Spectroscopy, via N. Carrara 1, Sesto F. No., 50019, Italy; Dipartimento di Scienze Biomediche Sperimentali e Cliniche “Mario Serio”, Università degli Studi di Firenze, Viale Morgagni 50, Firenze, 50134, Italy; Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Firenze, Viale Morgagni 63, Firenze, 50134, Italy; CNR-INO, via Nello Carrara 1, Sesto F. No., 50019, Italy; Dipartimento di Neuroscienze, Psicologia, Area del Farmaco e Salute del Bambino, Università degli Studi di Firenze, Viale Pieraccini, 6-50139, Firenze, Italy; Istituto Nazionale di Ricerca Metrologica (INRiM), Torino, 10135, Italy.
Abstract: The communication reports the use of liquid crystalline networks (LCNs) for engineering tissue cultures with human cells. Their ability as cell scaffolds for different cell lines is demonstrated. Preliminary assessments of the material biocompatibility are performed on human dermal fibroblasts and murine muscle cells (C2C12), demonstrating that coatings or other treatments are not needed to use the acrylate-based materials as support. Moreover, it is found that adherent C2C12 cells undergo differentiation, forming multinucleated myotubes, which show the typical elongated shape, and contain bundles of stress fibers. Once biocompatibility is demonstrated, the same LCN films are used as a substrate for culturing human induced pluripotent stem cell-derived cardiomyocites (hiPSC-CMs) proving that LCNs are capable to develop adult-like dimensions and a more mature cell function in a short period of culture in respect to standard supports. The demonstrated biocompatibility together with the extraordinary features of LCNs opens to preparation of complex cell scaffolds, both patterned and stimulated, for dynamic cell culturing. The ability of these materials to improve cell maturation and differentiation will be developed toward engineered heart and skeletal muscular tissues exploring regenerative medicine toward bioartificial muscles for injured sites replacement.
Volume: 13 (46) Pages from: 1702677 to: 1702677
More Information: The research leading to these results has received funding from the European Research Council under the European UnionKeyWords: cell scaffolds; fibroblast; human induced pluripotent stem cell-der; liquid crystalline networks; myoblastsDOI: 10.1002/smll.201702677Citations: 21data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2019-10-13References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here