Multimodal fiber-probe spectroscopy allows detecting
epileptogenic focal cortical dysplasia in children
Authors: Anand S., Cicchi R., Giordano F., Conti V., Buccoliero AM., Guerrini R., Pavone FS.
Autors Affiliation: National Institute of Optics-National Research Council (INO-CNR), Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy;
European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy;
Division of Neurosurgery, Department of Neuroscience I, Anna Meyer Children’s Hospital, University of Florence, Viale Pieraccini 24, 50141 Florence, Italy;
Pediatric Neurology, Neurogenetics and Neurobiology Unit and Laboratories, Neuroscience Department, Anna Meyer Children’s Hospital, University of Florence, Viale Pieraccini 24, 50139 Florence, Italy;
Division of Pathology, Anna Meyer Children’s Hospital, University of Florence, Viale Pieraccini 24, 50139 Florence, Italy;
Department of Physics, University of Florence, Via Giovanni Sansone 1, 50019 Sesto Fiorentino, Italy;
Abstract: We evaluated the diagnostic capability of a multimodal
spectroscopic approach for classifying normal brain tissue
and epileptogenic focal cortical dysplasia in children. We
employed fluorescence spectroscopy at two excitation wavelengths
(378 nm and 445 nm) and Raman spectroscopy
(at 785 nm excitation) for acquiring fluorescence and Raman
spectra from 10 normal brains, 16 focal cortical dysplasia
specimens and 1 cortical tuber tissue sites using a
custom-built multimodal optical point spectroscopic system.
We used principal component analysis combined with
leave-one-sample-out-cross-validation for tissue classification.
The study resulted in 100% sensitivity and 90% specificity
using the information obtained from fluorescence
at two distinct wavelengths and Raman spectroscopy for
discriminating normal brain tissue and focal cortical dysplasia.
Our results demonstrate that this methodology has
the potential to be applied clinically for the detection of
focal cortical dysplasia and can help to improve as precise
as possible surgical resection of the dysplastic tissue during
surgery for epilepsy.
Journal/Review: JOURNAL OF BIOPHOTONICS
KeyWords: focal cortical dysplasia; multimodal optical spectroscopy; principal component analysis