Nanostructured Brownian Surfaces Prepared through Two-Photon Polymerization: Investigation of Stem Cell Response
Authors: Marino A., Desii A., Pellegrino M., Pellegrini M., Filippeschi C., Mazzolai B., Mattoli V., Ciofani G.
Autors Affiliation: Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Pisa, Italy; The Biorobotics Institute, Scuola Superiore Sant’Anna, Viale Rinaldo Piaggio 34, 56025 Pontedera, Pisa, Italy; Dipartimento di Ricerca Traslazionale e delle Nuove Tecnologie in Medicina e Chirurgia University of Pisa, Via Savi 10, 56126 Pisa, Pisa, Italy; Scuola Normale Superiore di Pisa, Piazza dei Cavalieri 7, 56126 Pisa, Pisa, Italy
Abstract: Nondeterministic phenomena are at the base of plenty of biological processes that comprise physiological signaling, cellular communications, and biological architectures. Among them, natural surface topographies are often characterized by chaotic features that are not trivial to be recreated in vitro. Recently, some methods have been proposed to resemble the hierarchical organization of the extracellular microenvironment, through the chemical preparation of randomly rough and self-affine fractal surfaces. Notwithstanding, this approach does not allow the fractal dimension to be modulated at a desired value, being moreover the self-affinity maintained just for one decade of spatial frequencies. Here, we propose the replication of in silico generated Brownian surfaces through a two-photon polymerization technique. As a result of the direct laser writing of the desired patterns, we were able to obtain highly reproducible self-affine (in a range of two spatial frequency decades) structures characterized by the desired predetermined Hurst exponents. Rat mesenchymal stem cells were moreover cultured on the obtained substrates, highlighting interesting phenomena concerning cell adhesion, cytoskeleton conformation, and actin polymerization, strictly depending on the fractal dimension of the surfaces.
Journal/Review: ACS NANO
Volume: 8 (11) Pages from: 11869 to: 11882
KeyWords: two-photon polymerization; bio/non-bio interfaces; Brownian surfaces; mesenchymal stem cells; cytoskeleton organizationDOI: 10.1021/nn5052426Citations: 23data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2022-05-22References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here