Multimodal fiber probe spectroscopy for tissue diagnostics applications: a combined Raman-fluorescence approach

Year: 2014

Authors: Cicchi R., Anand S., Rossari S., Sturiale A., Giordano F., De Giorgi V., Maio V., Massi D., Nesi G., Buccoliero AM., Tonelli F., Guerrini R., Pimpinelli N., Pavone FS.

Autors Affiliation: National Institute of Optics, National Research Council (INO-CNR), Largo Enrico Fermi 6 – 50125, Florence, Italy;

European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Via Nello Carrara, 1 – 50019, Sesto Fiorentino, Italy;

Division of Clinical, Preventive and Oncology Dermatology, Department of Critical Care Medicine and Surgery, University of Florence, Piazza Indipendenza 11 – 50129, Florence, Italy;

Department of Clinical Physiopathology, Surgical Unit, University of Florence, Florence, Viale Giovanni Battista Morgagni 85 – 50134, Florence, Italy;

Division of Neurosurgery, Department of Neuroscience I, “Anna Meyer” Pediatric Hospital, Viale Gaetano Pieraccini 24 – 50141, Florence, Italy;

Division of Pathology, Department of Critical Care Medicine and Surgery, University of Florence, Viale Giovanni Battista Morgagni 85 – 50134, Florence, Italy;

Abstract: Two different optical fiber probes for combined Raman and fluorescence spectroscopic measurements were designed, developed and used for tissue diagnostics. Two visible laser diodes were used for fluorescence spectroscopy, whereas a laser diode emitting in the NIR was used for Raman spectroscopy. The two probes were based on fiber bundles with a central multimode optical fiber, used for delivering light to the tissue, and 24 surrounding optical fibers for signal collection. Both fluorescence and Raman spectra were acquired using the same detection unit, based on a cooled CCD camera, connected to a spectrograph. The two probes were successfully employed for diagnosing melanocytic lesions in a good agreement with common routine histology. The obtained results demonstrated that the multimodal approach is crucial for improving diagnostic capabilities. Further investigations were performed on colon and brain tissue samples in order to have a benchmark for diagnosing a broader range of tissue lesions and malignancies. The system presented here can improve diagnostic capabilities on a broad range of tissues and has the potential of being used for endoscopic inspections in the near future.

Conference title: Photonics West – BIOS 2014
Place: San Francisco, US

KeyWords: Fluorescence; Fluorescence spectroscopy; Histology; Industrial research; Multimode fibers; Probes; Vibrational spectroscopy, Diagnostic capabilities; Fluorescence and luminescence; Multi-modal approach; Multimode optical fibers; Raman; Spectroscopic measurements; Tissue characterization; Visible laser diodes, Tissue
DOI: 10.1117/12.2037024

Citations: 1
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