A combined Raman-fluorescence spectroscopic probe for tissue diagnostics applications

Year: 2013

Authors: Cicchi R., Cosci A., Rossari S., Sturiale A., Giordano F., De Giorgi V., Maio V., Massi D., Nesi G., Buccoliero AM., Tonelli F., Guerrini R., Pimpinelli N., Pavone FS.

Autors Affiliation: National Institute of Optics, National Research Council (INO-CNR), Largo Enrico Fermi 6 – 50125, Florence, Italy;
European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Via Nello Carrara, 1 – 50019, Sesto Fiorentino, Italy;

Division of Clinical, Preventive and Oncology Dermatology, Department of Critical Care Medicine and Surgery, University of Florence, Piazza Indipendenza 11 – 50129, Florence, Italy;

Department of Clinical Physiopathology, Surgical Unit, University of Florence, Florence, Viale Giovanni Battista Morgagni 85 – 50134, Florence, Italy;

Division of Neurosurgery, Department of Neuroscience I, “Anna Meyer” Pediatric Hospital, Viale Gaetano Pieraccini 24 – 50141, Florence, Italy;

Division of Pathology, Department of Critical Care Medicine and Surgery, University of Florence, Viale Giovanni Battista Morgagni 85 – 50134, Florence, Italy;

Department of Physics, University of Florence, Via Giovanni Sansone 1 – 50019, Sesto Fiorentino,

Abstract: We designed and developed two different optical fibre probes for combined Raman and fluorescence spectroscopic measurements on human tissues. The experimental setup combines fluorescence spectroscopy and Raman spectroscopy in a multimodal approach. Two laser diodes, respectively emitting in the UV (378 nm) and in the visible (445 nm), were used for fluorescence spectroscopy. An additional laser diode emitting in the NIR (785 nm) was used for Raman spectroscopy. Laser light was delivered to the tissue under examination through a multimode optical fibre located in the centre of the fibre bundle probe. The surrounding 24 optical fibres were used for collection of the signal of interest and for delivering light to a common detection unit. Both fluorescence and Raman spectra were acquired on a cooled CCD camera, connected to a spectrograph. The device was successfully used for diagnosing melanocytic lesions in a good agreement with common routine histology. Additional measurements were performed on other human tissue samples, such as colon tissue and brain tissue in order to test the capability of the device for diagnosing a broader range of tissue lesions and malignancies. The system has the potential to improve diagnostic capabilities on a broad range of tissues and to be used for endoscopic inspections in the near future.© 2013 OSA-SPIE.

Conference title: European Conference on Biomedical Optics – ECBO 2013
Place: Munich, Germany

KeyWords: Diagnostic capabilities; Fluorescence and luminescence; Multimode optical fibres; Optical fibre probes; Raman; Spectroscopic measurements; Spectroscopic probes; Tissue characterization, Fluorescence; Fluorescence spectroscopy; Histology; Light; Multimode fibers; Probes; Raman spectroscopy; Semiconductor lasers, Tissue
DOI: 10.1117/12.2031370