In-vivo imaging of psoriatic lesions with polarization multispectral dermoscopy and multiphoton microscopy

Year: 2014

Authors: Kapsokalyvas D., Cicchi R., Bruscino N., Alfieri D., Prignano F., Massi D., Lotti T., Pavone FS.

Autors Affiliation: European Laboratory for Non-Linear Spectroscopy (LENS) University of Florence, Sesto-Fiorentino, 50019, Italy;
National Institute of Optics, National Research Council (INO-CNR), 50125, Florence, Italy;
Division of Clinical, Preventive and Oncology Dermatology, Department of Critical Care Medicine and Surgery, University of Florence, 50129, Florence, Italy;
Light4tech Firenze srl, 50018, Scandicci, Italy

Department of Surgery and Translational Medicine, University of Florence, 50134, Florence, Italy

Chair of Dermatology and Venereology, University Guglielmo Marconi, 00193, Rome, Italy

Department of Physics, University of Florence, 50019, Sesto Fiorentino, Italy

Abstract: Psoriasis is a skin autoimmune disease characterized by hyperkeratosis, hyperproliferation of the epidermis and dilatation of dermal papillary blood vessels. Healthy skin (5 volunteers) and psoriatic lesions (3 patients) were visualized in vivo, with high contrast and resolution, with a Polarization Multispectral Dermoscope and a Multiphoton Microscope. Psoriatic features were identified and quantified. The effective diameter of the superficial blood vessels was measured at 35.2 +/- 7.2 mu m and the elongated dermal papillae had an effective diameter of 64.2 +/- 22.6 mu m. The methodologies developed could be employed for quantitative diagnostic purposes and furthermore serve as a monitoring method of the effect of personalized treatments. (C) 2014 Optical Society of America


Volume: 5 (7)      Pages from: 2405  to: 2419

More Information: The authors acknowledge the contribution of R. Ballerini, A. Hajeb (Mechanical Workshop of LENS), M. Giuntini, M. De Pas, A. Montuori (Electronic Workshop of LENS), on the microscope mechanics and electronics. The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreements no 228334 (OptBio) and 284464 (Bioptichal) and from the Italian Ministry for Education, University and Research in the framework of the Flagship Project NANOMAX. D. Kapsokalyvas gratefully acknowledges funding from Marie Curie Host Fellowships Action for Early Stage Research Training ATLAS programme (MEST-CT-2004-008048). Financial support by the Ente Cassa di Risparmio di Firenze (private foundation) is acknowledged.
KeyWords: Blood vessels; Polarization, Autoimmune disease; Dermal papilla; Effective diameter; High contrast; In-Vivo imaging; Monitoring methods; Multi-photon microscopy; Multi-spectral, Diagnosis, adult; article; autofluorescence imaging; blood vessel diameter; camera; case report; contrast; controlled study; epidermis; epiluminescence microscopy; female; human; illumination; image analysis; in vivo study; keratinocyte; laser; light emitting diode; light scattering; male; microscope; multiphoton microscopy; optical resolution; polarization multispectral dermoscopy; psoriasis; three dimensional imaging
DOI: 10.1364/BOE.5.002405

Citations: 29
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