Scientific Results

In vivo non-invasive monitoring of collagen remodelling by two-photon microscopy after micro-ablative fractional laser resurfacing

Year: 2014

Authors: Cicchi R., Kapsokalyvas D., Troiano M., Campolmi P., Morini C., Massi D., Cannarozzo G., Lotti T., Pavone FS.

Autors Affiliation: National Institute of Optics, National Research Council (INO-CNR), Largo E. Fermi 6, 50125, Florence, Italy;
European Laboratory for Non-linear Spectroscopy (LENS), Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy;
Division of Clinical, Preventive and Oncology Dermatology, Department of Critical Care Medicine and Surgery, University of Florence, Piazza Indipendenza, Florence, Italy;
Division of Pathology, Department of Surgery and Translational Medicine, University of Florence, Viale G.B. Morgagni 85,
Florence, Italy;
Chair of Dermatology and Venereology, University “Guglielmo Marconi”, Via Plinio 44, 00193, Rome, Italy;
Department of Physics, University of Florence, Via Giovanni Sansone 1, 50019, Sesto Fiorentino, Italy

Abstract: Non-linear optical microscopy is becoming popular as a non-invasive in vivo imaging modality in dermatology. In this study, combined TPF and SHG microscopy were used to monitor collagen remodelling in vivo after micro-ablative fractional laser resurfacing. Papillary dermis of living subjects, covering a wide age range, was imaged immediately before and forty days after treatment. A qualitative visual examination of acquired images demonstrated an age-dependent remodelling effect on collagen. Additional quantitative analysis of new collagen production was performed by means of two image analysis methods. A higher increase in SHG to TPF ratio, corresponding to a stronger treatment effectiveness, was found in older subjects, whereas the effect was found to be negligible in young, and minimal in middle age subjects. Analysis of collagen images also showed a dependence of the treatment effectiveness with age but with controversial results. While the diagnostic potential of in vivo multiphoton microscopy has already been demonstrated for skin cancer and other skin diseases, here we first successfully explore its potential use for a non-invasive follow-up of a laser-based treatment. ((c) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Journal/Review: JOURNAL OF BIOPHOTONICS

Volume: 7 (11-12)      Pages from: 914  to: 925

More Information: The authors thank R. Ballerini, A. Hajeb (Mechanical Workshop of LENS), M. Giuntini, M. De Pas, A. Montuori (Electronic Workshop of LENS), for their help in building microscope mechanics and electronics. The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreements number 228334 and 284464, and from the Italian Ministry for Education, University and Research in the framework of the Flagship Project NANOMAX. Furthermore, financial support by the Ente Cassa di Risparmio di Firenze (private foundation) is acknowledged.
DOI: 10.1002/jbio.201300124

Citations: 32
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