Holographic tracking of living cells by three-dimensional reconstructed complex wavefronts alignment

Year: 2014

Authors: Memmolo P., Miccio L., Finizio A., Netti P., Ferraro P.

Autors Affiliation: Center for Advanced Biomaterials for HealthCare@CRIB, Istituto Italiano di tecnologia, Largo Barsanti e Matteucci 53, 80125 Napoli, Italy;
CNR-Istituto Nazionale di Ottica, via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy

Abstract: We propose here a new three-dimensional (3D) holographic tracking method capable to track, simultaneously and in a single step, all the spatial coordinates of micro-objects. The approach is based on the enhanced correlation coefficient (ECC) maximization method but applied, for the first time to the best of our knowledge, directly on the holographic reconstructed complex wave fields. The key novelty of the proposed strategy is its ability to calculate simultaneously the 3D coordinates of cells, without decoupling the contribution of amplitude and phase. The proposed strategy is tested on living cells (i.e., NIH 3T3 mouse fibroblast) flowing into a microfluidic channel and compared with classical holographic tracking approach. Theoretical description and experimental validation of the proposed strategy are reported. (C) 2014 Optical Society of America

Journal/Review: OPTICS LETTERS

Volume: 39 (9)      Pages from: 2759  to: 2762

KeyWords: Digital holography; Three-dimensional microscopy; Pattern recognition; target tracking; Digital image processing
DOI: 10.1364/OL.39.002759

Citations: 23
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