3D visualization and biovolume estimation of motile cells by digital holography

Year: 2014

Authors: Merola F., Miccio L., Memmolo P., Di Caprio G., Coppola G., Netti P.

Autors Affiliation: CNR-INO

Abstract: For the monitoring of biological samples, physical parameters such as size, shape and refractive index are of crucial importance. However, up to now the morphological in-vitro analysis of in-vitro cells has been limited to 2D analysis by classical optical microscopy such as phase-contrast or DIC. Here we show an approach that exploits the capability of optical tweezers to trap and put in self-rotation bovine spermatozoa flowing into a microfluidic channel. At same time, digital holographic microscopy allows to image the cell in phase-contrast modality for each different angular position, during the rotation. From the collected information about the cell


Volume: 9129      Pages from: 91291W  to: 91291W

KeyWords: Digital Holography; optical tweezers; microfluidics; biovolume;
DOI: 10.1117/12.2052836

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