Multimodal nonlinear imaging of atherosclerotic plaques differentiation of triglyceride and cholesterol deposits

Year: 2014

Authors: Matthäus C., Cicchi R., Meyer T., Lattermann A., Schmitt M., Romeike B.F.M., Krafft C., Dietzek B., Brehm B.R., Pavone FS., Popp J.

Autors Affiliation: Leibniz Institute of Photonic Technology (IPHT-Jena), Albert Einstein Straße 9, 07745 Jena, Germany;
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743 Jena, Germany;
National Institute of Optics, National Research Council (INO-CNR), Largo E. Fermi 6 – 50125, Florence, Italy;
European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence Via Nello Carrara 1-50019, Sesto Fiorentino (Firenze), Italy;
Clinic for Internal Medicine, Jena University Hospital, Friedrich-Schiller-University, Erlanger Allee 101, 07747 Jena, Germany;
Institute of Pathology, Department of Neuropathology, Jena University Hospital – Friedrich-Schiller-University Erlanger Allee 101, 07740 Jena, Germany;
Catholic Clinic — Koblenz, Internal Medicine & Cardiology, Rudolf Virchow Str. 9, 56073 Koblenz, Germany

Abstract: Cardiovascular diseases in general and atherothrombosis as the most common of its individual disease entities is the leading cause of death in the developed countries. Therefore, visualization and characterization of inner arterial plaque composition is of vital diagnostic interest, especially for the early recognition of vulnerable plaques. Established clinical techniques provide valuable morphological information but cannot deliver information about the chemical composition of individual plaques. Therefore, spectroscopic imaging techniques have recently drawn considerable attention. Based on the spectroscopic properties of the individual plaque components, as for instance different types of lipids, the composition of atherosclerotic plaques can be analyzed qualitatively as well as quantitatively. Here, we compare the feasibility of multimodal nonlinear imaging combining two-photon fluorescence (TPF), coherent anti-Stokes Raman scattering (CARS) and second-harmonic generation (SHG) microscopy to contrast composition and morphology of lipid deposits against the surrounding matrix of connective tissue with diffraction limited spatial resolution. In this contribution, the spatial distribution of major constituents of the arterial wall and atherosclerotic plaques like elastin, collagen, triglycerides and cholesterol can be simultaneously visualized by a combination of nonlinear imaging methods, providing a powerful label-free complement to standard histopathological methods with great potential for in vivo application.


Volume: 7 (5)      Pages from: 1450027  to: 1450027

More Information: Financial support from the European Union via the Europaischer Fonds fur Regionale Entwicklung (EFRE) and the \”Thuringer Ministerium fur Bildung Wissenschaft und Kultur (TMBWK)\” (Projects: B714-07037, B578-06001, 14.90 HWP) and via the European network of excellence P4L (Photonics4Life) as well as financial support by the German Ministry for Science and Education (BMBF) MediCARS (FKZ: 13N10774) is highly acknowledged.
KeyWords: Cholesterol; Coherent scattering; Collagen; Deposits; Diagnosis; Diseases; Imaging techniques; Musculoskeletal system; Nonlinear optics; Raman scattering; Raman spectroscopy, Atherosclerosis; Cardio-vascular disease; Coherent anti Stokes Raman scattering; Diffraction limited spatial resolution; Morphological information; Nonlinear microscopy; Second harmonic generation microscopies (SHG); Spectroscopic imaging techniques, Harmonic generation
DOI: 10.1142/S1793545814500278

Citations: 5
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