Multimodal nonlinear microscopy: a powerful label-free method for supporting standard diagnostics on biological tissues

Year: 2014

Authors: Cicchi R., Pavone FS.

Autors Affiliation: National Institute of Optics, National Research Council (INO-CNR), I-50125 Florence, Italy; European Laboratory for Nonlinear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy; Department of Physics, University of Florence, Sesto Fiorentino, Italy; International Center of Computational Neurophoton ICON, Florence, Italy

Abstract: The large use of nonlinear laser scanning microscopy in the past decade paved the way for potential clinical application of this imaging technique. Modern nonlinear microscopy techniques offer promising label-free solutions to improve diagnostic performances on tissues. In particular, the combination of multiple nonlinear imaging techniques in the same microscope allows integrating morphological with functional information in a morpho-functional scheme. Such approach provides a high-resolution label-free alternative to both histological and immunohistochemical examination of tissues and is becoming increasingly popular among the clinical community. Nevertheless, several technical improvements, including automatic scanning and image analysis, are required before the technique represents a standard diagnostic method. In this review paper, we highlight the capabilities of multimodal nonlinear microscopy for tissue imaging, by providing various examples on colon, arterial and skin tissues. The comparison between images acquired using multimodal nonlinear microscopy and histology shows a good agreement between the two methods. The results demonstrate that multimodal nonlinear microscopy is a powerful label-free alternative to standard histopathological methods and has the potential to find a stable place in the clinical setting in the near future.


Volume: 7 (5)      Pages from: 1330008  to: 1330008

More Information: The research leading to these results has received funding: from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreements 228334 and 284464; from the Italian Ministry for Education, University and Research in the framework of the Flagship Project NANOMAX; from the Ente Cassa di Risparmio di Firenze (private foundation).
KeyWords: Histology; Imaging techniques; Tissue, Clinical application; Diagnostic performance; Functional information; Multimodal nonlinear microscopies; Non-linear imaging techniques; Nonlinear microscopy; Technical improvement; Tissue imaging, Tissue engineering
DOI: 10.1142/S1793545813300085

Citations: 12
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