4D tracking of clinical seminal samples for quantitative characterization of motility parameters

Year: 2014

Authors: Di Caprio G., El Mallahi A., Ferraro P., Dale R., Coppola G., Dale B., Coppola G., Dubois F.

Autors Affiliation: Institute for Microelectronics and Microsystems, Unit of Naples – National Research Council, Naples 80121, Italy; Microgravity Research Centre, Université Libre de Bruxelles, Brussels, 1050, Belgium; National Institute of Optics, Unit of Naples – National Research Council, Pozzuoli 80078, Italy; Center for Assisted Fertilization – CFA, Naples 80123, Italy

Abstract: In this paper we investigate the use of a digital holographic microscope, with partial spatial coherent illumination, for the automated detection and tracking of spermatozoa. This in vitro technique for the analysis of quantitative parameters is useful for assessment of semen quality. In fact, thanks to the capabilities of digital holography, the developed algorithm allows us to resolve in-focus amplitude and phase maps of the cells under study, independently of focal plane of the sample image. We have characterized cell motility on clinical samples of seminal fluid. In particular, anomalous sperm cells were characterized and the quantitative motility parameters were compared to those of normal sperm. (C) 2014 Optical Society of America


Volume: 5 (3)      Pages from: 690  to: 700

KeyWords: optics; holography; motile cells
DOI: 10.1364/BOE.5.000690

Citations: 53
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