4D tracking of clinical seminal samples for quantitative characterization of motility parameters
Authors: Di Caprio G., El Mallahi A., Ferraro P., Dale R., Coppola G., Dale B., Coppola G., Dubois F.
Autors Affiliation: Institute for Microelectronics and Microsystems, Unit of Naples – National Research Council, Naples 80121, Italy; Microgravity Research Centre, Université Libre de Bruxelles, Brussels, 1050, Belgium; National Institute of Optics, Unit of Naples – National Research Council, Pozzuoli 80078, Italy; Center for Assisted Fertilization – CFA, Naples 80123, Italy
Abstract: In this paper we investigate the use of a digital holographic microscope, with partial spatial coherent illumination, for the automated detection and tracking of spermatozoa. This in vitro technique for the analysis of quantitative parameters is useful for assessment of semen quality. In fact, thanks to the capabilities of digital holography, the developed algorithm allows us to resolve in-focus amplitude and phase maps of the cells under study, independently of focal plane of the sample image. We have characterized cell motility on clinical samples of seminal fluid. In particular, anomalous sperm cells were characterized and the quantitative motility parameters were compared to those of normal sperm. (C) 2014 Optical Society of America
Journal/Review: BIOMEDICAL OPTICS EXPRESS
Volume: 5 (3) Pages from: 690 to: 700
KeyWords: optics; holography; motile cellsDOI: 10.1364/BOE.5.000690Citations: 49data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2021-10-17References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here