Microwave Photochemical Reactor for the Online Oxidative Decomposition of p-Hydroxymercurybenzoate (pHMB)-Tagged Proteins and Their Determination by Cold Vapor Generation-Atomic Fluorescence Detection
Authors: Campanella B., Rivera G.J., Ferrari C., Biagi S., Onor M., D’Ulivo A., Bramanti E.
Autors Affiliation: National Research Council of Italy, C.N.R., Istituto di Chimica dei Composti Organo Metallici − ICCOM − UOS Pisa, Area di Ricerca, Via G. Moruzzi 1, 56124 Pisa, Italy; Chemical Engineering Department, University of Guanajuato, Noria Alta s/n 36050 Guanajuato, Gto., Mexico; National Research Council of Italy, C.N.R., Istituto Nazionale di Ottica, INO − UOS Pisa, Area di Ricerca, Via G. Moruzzi 1, 56124 Pisa, Italy
Abstract: A novel method is presented for the characterization and determination of thiolic proteins. After the labeling
with p-hydroxymercurybenzoate, the pHMB-labeled proteins underwent on-line oxidation with a novel microwave (MW)/
UV photochemical reactor, followed by cold vapor generationatomic fluorescence spectrometry (CVG-AFS) detection.
The MW/UV process led to the conversion of pHMB to Hg(II) with a yield of 89.0 ± 0.5% without using chemical oxidizing
reagents and avoiding the use of toxic carcinogenic compounds. Hg(II) was reduced to Hg0 in a knotted reaction
coil with NaBH4 solution, stripped from the solution by an argon flow and detected. The chromatographic method for
abeled thiolic peptides was linear in the 0.2−100 μmol L−1 range, with a LOD as mercury of 57 nmol L−1. This system has
proven to be a useful interface for liquid chromatography coupled with CVG-AFS in the determination and characterization
of thiolic proteins. This method has been applied to the determination of thiolic peptides after tryptic digestion of
serum albumins from different species (human, bovine, rat, horse, and sheep).
Journal/Review: ANALYTICAL CHEMISTRY
Volume: 85 (24) Pages from: 12152 to: 12157
KeyWords: Microwave; Protein; DOI: 10.1021/ac403389zCitations: 4data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2020-01-12References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here