Determination of thiomersal by flow injection coupled with microwave-assisted photochemical online oxidative decomposition of organic mercury and cold vapor atomic fluorescence spectroscopy
Authors: Campanella B., Onor M., Mascherpa M.C., D’Ulivo A., Ferrari C., Bramanti E.
Autors Affiliation: National Research Council of Italy, C.N.R., Istituto di Chimica dei Composti Organo Metallici-ICCOM-UOS Pisa, Area di Ricerca, Via G. Moruzzi 1, 56124 Pisa, Italy; National Research Council of Italy, C.N.R., Istituto Nazionale di Ottica, INO–UOS Pisa, Area di Ricerca, Via G. Moruzzi 1, 56124 Pisa, Italy
Abstract: We developed a flow injection (FI) method for the determination of thiomersal (sodium ethylmercurithiosalicylate, C9H9HgNaO2S) based on the UV/microwave (MW) photochemical, online oxidation of organic mercury, followed by cold vapor generation atomic fluorescence spectrometry (CVG-AFS) detection. Thiomersal was quantitatively converted in the MW/UV process to Hg(II), with a yield of 97 +/- 3%. This reaction was followed by the reduction of Hg(II) to Hg(0) performed in a knotted reaction coil with NaBH4 solution, and AFS detection in an Ar/H-2 miniaturized flame. The method was linear in the 0.01-2 mu g mL(-1) range, with a LOD of 0.003 mu g mL(-1). This method has been applied to the determination of thiomersal in ophthalmic solutions, with recoveries ranging between 97% and 101%. We found a mercury concentration in commercial ophthalmic solutions ranging between 7.5 and 59.0 mu g mL(-1). (C) 2013 Elsevier B.V. All rights reserved.
Journal/Review: ANALYTICA CHIMICA ACTA
Volume: 804 Pages from: 66 to: 69
More Information: DOI: 10.1016/j.aca.2013.10.018KeyWords: Thiomersal; Atomic fluorescence spectrometry; DOI: 10.1016/j.aca.2013.10.018Citations: 19data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2019-10-13References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here