Imaging through scattering microfluidic channels by digital holography for information recovery in lab on chip

Year: 2013

Authors: Bianco V., Paturzo M., Gennari O., Finizio A., Ferraro P.

Autors Affiliation: CNR Istituto Nazionale di Ottica—Sezione di Napoli, Via Campi Flegrei, 34 80078 Pozzuoli (Napoli), Italy

Abstract: We tackle the problem of information recovery and imaging through scattering microfluidic chips by means of digital holography (DH). In many cases the chip can become opalescent due to residual deposits settling down the inner channel faces, biofilm formation, scattering particle uptake by the channel cladding or its damaging by corrosive substances, or even by condensing effect on the exterior channels walls. In these cases white-light imaging is severely degraded and no information is obtainable at all about the flowing samples. Here we investigate the problem of counting and estimating velocity of cells flowing inside a scattering chip. Moreover we propose and test a method based on the recording of multiple digital holograms to retrieve improved phase-contrast images despite the strong scattering effect. This method helps, thanks to DH, to recover information which, otherwise, would be completely lost. (C)2013 Optical Society of America

Journal/Review: OPTICS EXPRESS

Volume: 21 (20)      Pages from: 23985  to: 23996

More Information: This work was supported by Progetto Bandiera \”La Fabbrica del Futuro\” in the framework of the funded project \”Plastic lab-on-chips for the optical manipulation of single cells\” (PLUS).
DOI: 10.1364/OE.21.023985

Citations: 38
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