Combined fluorescence-Raman spectroscopic setup for the diagnosis of melanocytic lesions

Year: 2014

Authors: Cicchi R., Cosci A., Rossari S., Kapsokalyvas D., Baria E., Maio V., Massi D., De Giorgi V., Pimpinelli N., Pavone FS.

Autors Affiliation: National Institute of Optics, National Research Council (INO-CNR), Largo E. Fermi 6, 50125, Florence, Italy; European Laboratory for Non-linear Spectroscopy (LENS), Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy; Division of Clinical, Preventive and Oncology Dermatology, Department of Critical Care Medicine and Surgery, University of Florence, Piazza Indipendenza 11, 50129 Florence, Italy; Department of Physics, University of Florence, Via G. Sansone 1, 50019, Sesto Fiorentino, Italy; Division of Pathology, Department of Critical Care Medicine and Surgery, University of Florence, Viale GB Morgagni 85, 50134, Florence, Italy; International Center of Computational Neurophotonics (ICON), Via Nello Carrara 1, 50019 Florence, Italy

Abstract: Two optical fibre-based probes for spectroscopic measurements on human tissues were designed and developed. The two probes combine fluorescence and Raman spectroscopy in a multimodal approach. The fluorescence excitation was provided by two laser diodes emitting in the UV (378 nm) and in the visible (445 nm) range, while a third source in the NIR (785 nm) was used for Raman. The device was tested on freshly excised human skin biopsies clinically diagnosed as malignant melanoma, melanocytic nevus, or healthy skin. Discrimination of lesions based on their fluorescence and Raman spectra showed good correlation with the subsequent histological examination. ((c) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)


Volume: 7 (1-2)      Pages from: 86  to: 95

More Information: The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreements number 228334 and 284464, and from the Italian Ministry for Education, University and Research in the framework of the Flagship Project NANOMAX. Furthermore, financial support by Ente Cassa di Risparmio di Firenze (private foundation) is acknowledged.
KeyWords: Fluorescence excitation; Good correlations; Histological examination; Human tissues; Malignant melanoma; Melanocytic lesion; Multi-modal approach; Spectroscopic measurements, Dermatology; Fluorescence spectroscopy; Optical fibers; Probes; Raman spectroscopy, Fluorescence, glass fiber, adult; aged; algorithm; article; dermatology; differential diagnosis; equipment; female; fluorescence spectroscopy; human; male; melanoma; methodology; middle aged; pathology; pigmented nevus; Raman spectrometry; skin; spectrofluorometry; young adult, dermatology; fluorescence spectroscopy; Raman spectroscopy, Adult; Aged; Algorithms; Diagnosis, Differential; Female; Humans; Male; Melanoma; Middle Aged; Nevus, Pigmented; Optical Fibers; Skin; Spectrometry, Fluorescence; Spectrum Analysis, Raman; Young Adult
DOI: 10.1002/jbio.201200230

Citations: 45
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