Double optical fibre-probe device for the diagnosis of melanocytic lesions

Year: 2012

Authors: Cicchi R., Cosci A., Rossari S., De Giorgi V., Kapsokalyvas D., Massi D., Pavone FS.

Autors Affiliation: European Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy;
Department of Dermatological Sciences, University of Florence, Florence, Italy;
Department of Surgical and Medical Critical Care, Division of Pathology, University of Florence, Florence, Italy

Abstract: We have designed and developed an optical fiber-probe for spectroscopic measurements on human tissues. The experimental setup combines fluorescence spectroscopy and Raman spectroscopy in a multidimensional approach. Concerning fluorescence spectroscopy, the excitation is provided by two laser diodes, one emitting in the UV (378 nm) and the other emitting in the visible (445 nm). These two lasers are used to selectively excite fluorescence from NADH and FAD, which are among the brightest endogenous fluorophores in human tissues. For Raman and NIR spectroscopy, the excitation is provided by a third laser diode with 785 nm excitation wavelength. Laser light is delivered to the tissue through the central optical fiber of a fiber bundle. The surrounding 48 fibers of the bundle are used for collecting fluorescence and Raman and for delivering light to the spectrograph. Fluorescence and Raman spectra are acquired on a cooled CCD camera. The instrument has been tested on fresh human skin biopsies clinically diagnosed as malignant melanoma, melanocytic nevus, or healthy skin, finding an optimal correlation with the subsequent histological exam. In some cases our examination was not in agreement with the clinical observation, but it was with the histological exam, demonstrating that the system can potentially contribute to improve clinical diagnostic capabilities and hence reduce the number of unnecessary biopsies.

Conference title: Photonics Europe 2012
Place: Bruxelles, Belgium

More Information: The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement 228334. This research project has been also supported by Human Frontier Science Program (HFSP) under grant agreement RGP0027/2009.
KeyWords: Clinical diagnostics; Clinical observation; Cooled CCD; Excitation wavelength; Experimental setup; Fiber bundles; Human skin; Human tissues; Laser lights; Malignant melanoma; Melanocytic lesion; Multi-dimensional approach; NIR spectroscopy; Optimal correlation; Raman; Spectroscopic measurements, Biopsy; Dermatology; Fluorescence; Fluorescence spectroscopy; Health care; Histology; Near infrared spectroscopy; Optical fibers; Photonics; Probes; Raman scattering; Raman spectroscopy; Semiconductor lasers; Spectroscopy; Tissue, Fluorophores
DOI: 10.1117/12.922405

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