Time- and Spectral-resolved two-photon imaging of healthy bladder mucosa and carcinoma in situ
Authors: Cicchi R., Crisci A., Cosci A., Nesi G., Kapsokalyvas D., Giancane S., Carini M., Pavone F.S.
Autors Affiliation: LENS, European Laboratory for Non-Linear Spectroscopy, Via Nello Carrara 1, Sesto Fiorentino, I-50019, Italy; University of Florence Medical School, Department of Surgical and Medical Critical Area, University of Florence, Florence, I-50100, Italy; Division of Urology, Department of Surgical and Medical Critical Area, University of Florence, Florence, I-50100, Italy
Abstract: Combined non-linear imaging techniques were used to deeply image human ex-vivo fresh biopsies of bladder as well as to discriminate between healthy bladder mucosa and carcinoma in situ. Morphological examination by two-photon excited fluorescence and second-harmonic generation has shown a good agreement with corresponding common routine histology performed on the same samples. Tumor cells appeared slightly different in shape and with a smaller cellular-to-nuclear dimension ratio with respect to corresponding normal cells. Further differences between the two tissue types were found in both spectral emission and fluorescence lifetime distribution by performing temporal-and spectral-resolved analysis of fluorescence. This method may represent a promising tool to be used in a multi-photon endoscope, in a confocal endoscope or in a spectroscopic probe for in-vivo optical diagnosis of bladder cancer. (C)2010 Optical Society of America
Journal/Review: OPTICS EXPRESS
Volume: 18 (4) Pages from: 3840 to: 3849
KeyWords: Fluorescence; Imaging techniques, Bladder cancers; Ex-vivo; Fluorescence lifetimes; In-situ; In-vivo; Multiphotons; Non-linear imaging techniques; Optical Diagnosis; Second harmonic generation; Spectral emission; Spectroscopic probes; Tissue types; Tumor cells; Two photon imaging; Two-photon excited fluorescence, Photons, article; bladder; bladder tumor; human; image enhancement; methodology; mucosa; multiphoton microscopy; pathology, Humans; Image Enhancement; Microscopy, Fluorescence, Multiphoton; Mucous Membrane; Urinary Bladder; Urinary Bladder NeoplasmsDOI: 10.1364/OE.18.003840Citations: 55data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2021-07-25References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here