Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging

Year: 2009

Authors: Matteini P., Ratto F., Rossi F., Cicchi R., Stringari C., Kapsokalyvas D., Pavone F.S., Pini R.

Autors Affiliation: Istituto di Fisica Applicata “Nello Carrara”, Consiglio Nazionale delle Ricerche, Via Madonna del Piano 10, Sesto Fiorentino, I-50019, Italy;
LENS, European Laboratory for Non-Linear Spectroscopy, Via Nello Carrara 1, Sesto Fiorentino, I-50019, Italy

Abstract: The loss of organization of the corneal collagen lattice induced by photothermal effects was analyzed by using second-harmonic generation (SHG) imaging. Porcine cornea samples were treated with low-power laser irradiation in order to get localized areas of tissue disorganization. The disorder induced within the irradiated area of corneal stroma was quantified by means of Discrete Fourier Transform, auto-correlation and entropy analyses of the SHG images. Polarization modulated SHG measurements allowed to probe the changes in the structural anisotropy of sub-micron hierarchical levels of the stromal collagen. Our results emphasize the great potential of the SHG imaging to detect subtle modifications in the collagen assembly. The proposed analytical methods may be used to track several genetic, pathologic, accidental or surgical-induced disorder states of biological tissues. (C) 2008 Optical Society of America

Journal/Review: OPTICS EXPRESS

Volume: 17 (6)      Pages from: 4868  to: 4878

KeyWords: Collagen; Discrete Fourier transforms; Nonlinear optics; Tissue, Analytical method; Biological tissues; Entropy analysis; Hierarchical level; Photothermal effects; Polarization-modulated; Porcine corneas; Structural anisotropy, Harmonic generation, collagen, animal; article; cornea; cytology; Fourier analysis; laser; light; radiation exposure; swine; temperature; ultrastructure, Animals; Collagen; Cornea; Fourier Analysis; Lasers; Light; Sus scrofa; Temperature
DOI: 10.1364/OE.17.004868

Citations: 133
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