Scientific Results

A new iterative Fourier transform algorithm for optimal design in holographic optical tweezers

Year: 2012

Authors: Memmolo P., Miccio L., Merola F., Ferraro P., Netti P.A.

Autors Affiliation: CNR – Istituto Nazionale di Ottica, via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy;
Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, P.le Tecchio 80, 80125 Naples, Italy

Abstract: We propose a new Iterative Fourier Transform Algorithm (IFTA) capable to suppress ghost traps and noise in Holographic Optical Tweezers (HOT), maintaining a high diffraction efficiency in a computational time comparable with the others iterative algorithms. The process consists in the planning of the suitable ideal target of optical tweezers as input of classical IFTA and we show we are able to design up to 4 real traps, in the field of view imaged by the microscope objective, using an IFTA built on fictitious phasors, located in strategic positions in the Fourier plane. The effectiveness of the proposed algorithm is evaluated both for numerical and optical reconstructions and compared with the other techniques known in literature.

Journal/Review: PROCEEDINGS OF SPIE

Volume: 8429      Pages from: 84296F  to: 84296F

KeyWords: Holographic Optical Tweezers
DOI: 10.1117/12.922927

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English