From molecular structure to tissue architecture: collagen organization probed by SHG microscopy
Authors: Cicchi R., Vogler N., Kapsokalyvas D., Dietzek B., Popp J., Pavone FS.
Autors Affiliation: National Institute of Optics, National Research Council (INO-CNR), Largo E. Fermi 6, 50125 Florence, Italy; Institute of Photonic Technology Jena (IPHT), Albert Einstein Straße 9, 07745 Jena, Germany; European Laboratory for Non-linear Spectroscopy (LENS), Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy; Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University, Helmholtzweg 4, 07743 Jena, Germany; Department of Physics, University of Florence, Via G. Sansone 1, 50019 Sesto Fiorentino, Italy
Abstract: Second-harmonic generation (SHG) microscopy is a fantastic tool for imaging collagen and probing its hierarchical organization from molecular scale up to tissue architectural level. In fact, SHG combines the advantages of a non-linear microscopy approach with a coherent modality able to probe molecular organization. In this manuscript we review the physical concepts describing SHG from collagen, highlighting how this optical process allows to probe structures ranging from molecular sizes to tissue architecture, through image pattern analysis and scoring methods. Starting from the description of the most relevant approaches employing SHG polarization anisotropy and forward backward SHG detection, we then focus on the most relevant methods for imaging and characterizing collagen organization in tissues through image pattern analysis methods, highlighting advantages and limitations of the methods applied to tissue imaging and to potential clinical applications. ((c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
Journal/Review: JOURNAL OF BIOPHOTONICS
Volume: 6 (2) Pages from: 129 to: 142
KeyWords: SHG microscopy; tissue imaging; collagenDOI: 10.1002/jbio.201200092Citations: 95data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2019-08-18References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here