Scientific Results

Dynamic Differential Image Contrast by Digital Holography for imaging and Quantitative Phase Microscopy

Year: 2011

Authors: Miccio L., Finizio A., Puglisi R., Balduzzi D., Galli A., Ferraro P.

Autors Affiliation: CNR – Istituto Nazionale di Ottica, Naples Section, Via Campi Flegrei,34 80078 Pozzuoli – Napoli Italy; Istituto Sperimentale Italiano “Lazzaro Spallanzani”, Località La Quercia, 26027—Rivolta d’Adda, Cremona, Italy

Abstract: Numerical managing and manipulation of complex wavefronts is performed to retrieve Differential Image Contrast (DIC) visualization starting from Digital Holographic (DH) recording. Dynamical Differential Holographic Image Contrast (DDHIC) is the name of the proposed technique. It dispenses from special optics and/or complex setup configurations with moveable components, as usually occurs in classical DIC. DDHIC presents as an appropriate method for investigating objects experiencing dynamic evolution during the measurement. In fact, it will be proved that it’s useful for floating samples since it allows, from a single recording, to set a-posteriori the best conditions for DIC imaging. Dynamic representation of phase-contrast along all directions will be presented improving the specimen visualization. Furthermore, it is useful for making the proper choice of DIC key parameters as the amount of shear and the bias, with the aim to optimize the visualized phase-contrast imaging. Investigation is carried out on different biological samples.

Journal/Review: PROCEEDINGS OF SPIE

Volume: 8086      Pages from: 80860D  to: 80860D

KeyWords: Digital holography; Interference Microscopy; Image enhancement
DOI: 10.1117/12.889875

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English