Coherent white light confocal fluorescence imaging and fluorescence lifetime imaging microscopy
Authors: Soria S., Quercioli F., Mercatelli R., Bianco F., Cacciari I., Pelli S., Righini G.C.
Autors Affiliation: Centro Studi e Ricerche “E. Fermi”, P. Del Viminale 2, 00184 Rome, Italy; IFAC-CNR, Istituto di Fisica Applicata “N. Carrara”, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy; ISC-CNR, Istituto dei Sistemi Complessi, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy
Abstract: We report on the application of a simple white light source based on the supercontinuum generation from commercial photonic crystal fibres to confocal fluorescence microscopy and fluorescence lifetime imaging (FLIM) microscopy. The coherent white light can be tuned by varying the wavelength and intensity of the pump, a Ti:Sapphire laser. There are several advantages jn the use of SC sources: spatially coherent white radiation, tuning ranges of approximately 400 nm, high brightness, a robust compact system (potentially all-fibre) and relatively low cost. Being pulsed, SC sources are suitable for FLIM and may be used for multiple excitation.
KeyWords: Compact system; Confocal fluorescence imaging; Confocal fluorescence microscopy; Fluorescence lifetime imaging; Fluorescence lifetime imaging (FLIM); Fluorescence lifetime imaging microscopy; High brightness; Low costs; Multiple excitations; Photonic crystal fibres; Supercontinuum (SC); Supercontinuum generations; Ti: Sapphire laser; Tuning ranges; White light; White light sources, Confocal microscopy; Corundum; Crystal whiskers; Fibers; Fluorescence; Fluorescence microscopy; Light; Light sources; Photonic crystals; Pumping (laser), Photonic crystal fibersDOI: 10.1117/12.821199Citations: 1data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2021-10-10References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here