Scientific Results

Coherent white light confocal fluorescence imaging and fluorescence lifetime imaging microscopy

Year: 2009

Authors: Soria S., Quercioli F., Mercatelli R., Bianco F., Cacciari I., Pelli S., Righini G.C.

Autors Affiliation: Centro Studi e Ricerche “E. Fermi”, P. Del Viminale 2, 00184 Rome, Italy; IFAC-CNR, Istituto di Fisica Applicata “N. Carrara”, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy; ISC-CNR, Istituto dei Sistemi Complessi, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy

Abstract: We report on the application of a simple white light source based on the supercontinuum generation from commercial photonic crystal fibres to confocal fluorescence microscopy and fluorescence lifetime imaging (FLIM) microscopy. The coherent white light can be tuned by varying the wavelength and intensity of the pump, a Ti:Sapphire laser. There are several advantages jn the use of SC sources: spatially coherent white radiation, tuning ranges of approximately 400 nm, high brightness, a robust compact system (potentially all-fibre) and relatively low cost. Being pulsed, SC sources are suitable for FLIM and may be used for multiple excitation.

Conference title:
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KeyWords: Compact system; Confocal fluorescence imaging; Confocal fluorescence microscopy; Fluorescence lifetime imaging; Fluorescence lifetime imaging (FLIM); Fluorescence lifetime imaging microscopy; High brightness; Low costs; Multiple excitations; Photonic crystal fibres; Supercontinuum (SC); Supercontinuum generations; Ti: Sapphire laser; Tuning ranges; White light; White light sources, Confocal microscopy; Corundum; Crystal whiskers; Fibers; Fluorescence; Fluorescence microscopy; Light; Light sources; Photonic crystals; Pumping (laser), Photonic crystal fibers
DOI: 10.1117/12.821199

Citations: 1
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