Dermal matrix scaffold engineered with adult mesenchymal stem cells and platelet-rich plasma as a potential tool for tissue repair and regeneration

Year: 2012

Authors: Formigli L., Benvenuti S., Mercatelli R., Quercioli F., Tani A., Mirabella C., Dama A., Saccardi R., Mazzanti B., Cellai I., Zecchi-Orlandini S.

Autors Affiliation: Department of Anatomy, Histology and Forensic Medicine, University of Florence, Italy;
Department of Clinical Physiopathology, University of Florence, Italy;
Institute of Complex Systems, National Council of Research, Florence, Italy;
Immunohaematology and Transfusion Medicine, University Hospital of Careggi, Florence, Italy
Department of Haematology, Cord Blood Bank, University Hospital of Careggi, Florence, Italy

Abstract: The purpose of this study was to investigate the efficacy of Integra (R), an artificial dermal matrix used as a dermal template for skin regeneration, to form a multifunctional scaffold with human bone marrow-derived mesenchymal stem cells (hMSCs) and platelet-rich plasma (PRP) for tissue engineering and regenerative technology. First, we showed that PRP, used as a supplement for growth medium, represented an optimal substitute for animal serum as well as a source of multiple growth factors, was able to satisfactorily support cell viability and cell proliferation and influence stemness gene expression in hMSCs. Moreover, Integra appeared to be a suitable substrate for hMSCs colonization, as judged by two-photon microscopy combined with fluorescence lifetime imaging (FLIM) and confocal analysis. The cells were then seeded on Integra + PRP for 24 and 48 h. Notably, in these conditions, the seeded cells exhibited a greater aptitude to colonize the scaffold, showed improved cell adhesion and spreading as compared with those cultured on Integra alone, and acquired a fibroblast-like phenotype, indicating that the bioengineered scaffold provided an appropriate environment for cellular growth and differentiation. In conclusion, these results, although preliminary, provide clues for the design of new therapeutic strategies for skin regeneration, consisting in the combination of mesenchymal stem cells with engineered biomaterials. Copyright (C) 2011 John Wiley & Sons, Ltd.


Volume: 6 (2)      Pages from: 125  to: 134

More Information: This study was partially supported by a grant from Ente Cassa di Risparmio di Firenze to S.Z.-O. and from the Italian Ministry of Universities to S.Z.-O. and L. F. Assistance in confocal imaging from Daniele Nosi and the supervision of Alessandro Peri are gratefully acknowledged.
KeyWords: Biomaterials; Growth factors; Mesenchymal stem cells; Multiphoton microscopy; Platelet-rich plasma; Regenerative medicine; Tissue regeneration; Tissue repair, Adult; Adult Stem Cells; Cell Count; Cell Proliferation; Cell Separation; Cell Survival; Cells, Cultured; Dermis; Extracellular Matrix; Gene Expression Regulation; Humans; Mesenchymal Stem Cells; Microscopy, Confocal; Microscopy, Fluorescence, Multiphoton; Platelet-Rich Plasma; Regeneration; Tissue Engineering; Tissue Scaffolds; Transcription Factors; Wound Healing
DOI: 10.1002/term.405

Citations: 24
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