Scientific Results

Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction

Year: 2010

Authors: Coppola G., Di Caprio G., Gioffrè M., Puglisi R., Balduzzi D., Galli A., Miccio L., Paturzo M., Grilli S., Finizio A., Ferraro P.

Autors Affiliation: CNR—Istituto per la Microelettronica e Microsistemi, Via Pietro Castellino 111, 80131—Napoli, Italy;
Istituto Sperimentale Italiano “Lazzaro Spallanzani”, Località La Quercia, 26027—Rivolta d’Adda, Cremona, Italy
CNR — Istituto Nazionale di Ottica, Sezione di Napoli, Via Campi Flegrei 34, 80078—Pozzuoli, Napoli, Italy

Abstract: A completely numerical method, named digital self-referencing holography, is described to easily accomplish a quantitative phase microscopy for microfluidic devices by a digital holographic microscope. The approach works through an appropriate numerical manipulation of the retrieved complex wavefront. The self-referencing is obtained by folding the retrieved wavefront in the image plane. The folding operation allows us to obtain the correct phase map by subtracting from the complex region of interest a flat area outside the microfluidic channel. To demonstrate the effectiveness of the method, quantitative phase maps of bovine spermatozoa and in vitro cells are retrieved. (C) 2010 Optical Society of America

Journal/Review: OPTICS LETTERS

Volume: 35 (20)      Pages from: 3390  to: 3392

More Information: This work was supported in part by the project SESSIBOV 9106/7303/2009 of the Ministero delle Politiche Agricole e Forestali.
DOI: 10.1364/OL.35.003390

Citations: 58
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English