Supercontinuum source tuned by an on-axis monochromator for fluorescence lifetime imaging
Authors: Mercatelli R., Soria S., Molesini G., Bianco F., Righini G., Quercioli F.
Autors Affiliation: CNR – Istituto di Fisica Applicata “N. Carrara”, Sesto Fiorentino; CNR – Istituto dei Sistemi Complessi, Sesto Fiorentino; CNR – Istituto Nazionale di Ottica, Firenze
Abstract: We report on the application of an optically tunable coherent white light source, based on the supercontinuum generation from microstructured optical fibres, to fluorescence lifetime imaging (FLIM) microscopy and Foerster resonance energy transfer (FRET). A prototype lens based on monotonic longitudinal chromatic aberration is used for tuning the supercontinuum wavelengths from 400 to 1000 nm and acts as an axial monochromator, suitable for fibre delivery in confocal microscopy. (C) 2010 Optical Society of America
Journal/Review: OPTICS EXPRESS
Volume: 18 (19) Pages from: 20505 to: 20511
More Information: The European NoE \”Photonics for Life\” (P4L) is acknowledged. F. Quercioli and R. Mercatelli gratefully acknowledge the funding of Ente Cassa di Risparmio of Florence, Italy. HEK cells were generously provided by Prof. A. Arcangeli. Useful discussions with G. Nunzi Conti and S. Pelli are also gratefully acknowledged. F. Bianco is now at the University of Trento, Italy.KeyWords: Confocal microscopy; Energy transfer; Light sources; Monochromators; Optical fibers, Chromatic aberration; Fluorescence lifetime imaging; Foerster resonance energy transfers; Microstructured; Optical fibre; Supercontinuum; Supercontinuum generations; Supercontinuum sources; White light sources; Fluorescence, article; equipment; Equipment design; Fluorescence microscopy; Fluorescence resonance energy transfer; Illumination; Instrumentation; Equipment Design; Equipment Failure Analysis; Fluorescence Resonance Energy Transfer; Lighting; Microscopy; FluorescenceDOI: 10.1364/OE.18.020505Citations: 16data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2020-09-13References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here