Scientific Results

Supercontinuum source tuned by an on-axis monochromator for fluorescence lifetime imaging

Year: 2010

Authors: Mercatelli R., Soria S., Molesini G., Bianco F., Righini G., Quercioli F.

Autors Affiliation: CNR – Istituto di Fisica Applicata “N. Carrara”, Sesto Fiorentino; CNR – Istituto dei Sistemi Complessi, Sesto Fiorentino; CNR – Istituto Nazionale di Ottica, Firenze

Abstract: We report on the application of an optically tunable coherent white light source, based on the supercontinuum generation from microstructured optical fibres, to fluorescence lifetime imaging (FLIM) microscopy and Foerster resonance energy transfer (FRET). A prototype lens based on monotonic longitudinal chromatic aberration is used for tuning the supercontinuum wavelengths from 400 to 1000 nm and acts as an axial monochromator, suitable for fibre delivery in confocal microscopy. (C) 2010 Optical Society of America

Journal/Review: OPTICS EXPRESS

Volume: 18 (19)      Pages from: 20505  to: 20511

More Information: The European NoE \”Photonics for Life\” (P4L) is acknowledged. F. Quercioli and R. Mercatelli gratefully acknowledge the funding of Ente Cassa di Risparmio of Florence, Italy. HEK cells were generously provided by Prof. A. Arcangeli. Useful discussions with G. Nunzi Conti and S. Pelli are also gratefully acknowledged. F. Bianco is now at the University of Trento, Italy.
KeyWords: Confocal microscopy; Energy transfer; Light sources; Monochromators; Optical fibers, Chromatic aberration; Fluorescence lifetime imaging; Foerster resonance energy transfers; Microstructured; Optical fibre; Supercontinuum; Supercontinuum generations; Supercontinuum sources; White light sources; Fluorescence, article; equipment; Equipment design; Fluorescence microscopy; Fluorescence resonance energy transfer; Illumination; Instrumentation; Equipment Design; Equipment Failure Analysis; Fluorescence Resonance Energy Transfer; Lighting; Microscopy; Fluorescence
DOI: 10.1364/OE.18.020505

Citations: 16
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