Oligonucletides direct syntheis on porous silicon chip

Year: 2008

Authors: De Stefano L., De Tommasi E., Rea I., Rotiroti L., Giangrande L., Oliviero G., Borbone N., Galeone A., Piccialli G.

Autors Affiliation: Istituto per la Microelettronica e Microsistemi – Unità di Napoli, Consiglio Nazionale delle Ricerche, via P. Castellino 111, I-80131 Napoli, Italy; Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli “Federico II”, Via D. Montesano 49, I-80131 Napoli, Italy

Abstract: A solid phase oligonucleotide (ON) synthesis on porous silicon (PSi) chip is presented. The prepared Si-OH surface were analyzed by FT-IR and the OH functions were quantified by reaction with 3’-phosphoramidite nucleotide building block. Short ONs were synthesized on the chip surface and the coupling yields evaluated.


Volume: 52      Pages from: 721  to: 722

KeyWords: Oligonucleotids; Porous Silicon;