Scientific Results

Direct full compensation of the aberrations in quantitative phase microscopy of thin objects by a single digital hologram

Year: 2007

Authors: Miccio L., Alfieri D., Grilli S., Ferraro P., Finizio A., De Petrocellis L., De Nicola S.

Autors Affiliation: CNR – Istituto Nazionale di Ottica Applicata, Via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy;
Istituto di Cibernetica “E. Caianiello” del CNR, Via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy

Abstract: Aberrations and the distortions due to the imaging optics can be compensated in quantitative phase microscopy of thin phase objects by digital holography using a single hologram. The reconstructed quantitative phase microscopy phase distribution map can be directly corrected in the reconstructed image plane by a numerical method. To remove this unwanted aberration, in the special case of thin objects, the authors perform a two-dimensional fit with the Zernike polynomials of the reconstructed unwrapped phase. Subtraction of the fitted polynomial from the original phase map gives quantitative phase microscopy phase map free of aberrations. (c) 2007 American Institute of Physics.


Volume: 90 (4)      Pages from: 041104-1  to: 041104-3

KeyWords: Holography; Image Processing; Optical Aberrations
DOI: 10.1063/1.2432287

Citations: 106
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