Dysplasia and tumor discrimination in brain tissues by combined fluorescence, Raman, and diffuse reflectance spectroscopies

Year: 2023

Authors: Baria E., Giordano F., Guerrini R., Caporalini C., Buccoliero A.M., Cicchi R., Pavone F.S.

Autors Affiliation: National Institute of Optics, National Research Council, Via Nello Carrara 1, Sesto Fiorentino 50019, Italy
European Laboratory for Non-Linear Spectroscopy, University of Florence, Via Nello Carrara 1, Sesto
Fiorentino 50019, Italy
Division of Neurosurgery, Department of Neuroscience I, “A. Meyer” Children’s Hospital, Viale Gaetano
Pieraccini 24, Florence 50141, Italy
Division of Pathology, Department of Critical Care Medicine and Surgery, University of Florence, Viale
Giovanni Battista Morgagni 85, Florence 50134, Italy
Department of Physics and Astrophysics, University of Florence, Via Sansone 1, Sesto Fiorentino 50019,
Italy

Abstract: Identification of neoplastic and dysplastic brain tissues is of paramount importance for improving the outcomes of neurosurgical procedures. This study explores the combined application of fluorescence, Raman and diffuse reflectance spectroscopies for the detection and classification of brain tumor and cortical dysplasia with a label-free modality. Multivariate analysis was performed to evaluate classification accuracies of these techniques–employed both in individual and multimodal configuration–obtaining high sensitivity and specificity. In particular, the proposed multimodal approach allowed discriminating tumor/dysplastic tissues against control tissue with 91%/86% sensitivity and 100%/100% specificity, respectively, whereas tumor from dysplastic tissues were discriminated with 89% sensitivity and 86% specificity. Hence, multimodal optical spectroscopy allows reliably differentiating these pathologies using a non-invasive, label-free approach that is faster than the gold standard technique and does not require any tissue processing, offering the potential for the clinical translation of the technology.

Journal/Review: BIOMEDICAL OPTICS EXPRESS

Volume: 14      Pages from: 1256  to: 1275

KeyWords: fluorescence spectroscopy, Raman spectroscopy, diffuse reflectance spectroscopy, brain tumor
DOI: 10.1364/BOE.477035

Citations: 2
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