Mineralization of 3D osteogenic model based on gelatin-dextran hybrid hydrogel scaffold bioengineered with mesenchymal stromal cells: A multiparametric evaluation
Year: 2021
Authors: Re F.; Sartore L.; Borsani E.; Ferroni M.; Baratto C.; Mahajneh A.; Smith A.; Dey K.; Almici C.; Guizzi P.; Bernardi S.; Faglia G.; Magni F.; Russo D.
Autors Affiliation: Bone Marrow Transplant Unit, Department of Clinical and Experimental Sciences, University of Brescia, ASST Spedali Civili, Piazzale Spedali Civili 1, Brescia, 25123, Italy; Centro di Ricerca Emato-Oncologica AIL (CREA), ASST Spedali Civili, Piazzale Spedali Civili 1, Brescia, 25123, Italy; Department of Mechanical and Industrial Engineering, University of Brescia, Via Branze 38, Brescia, 25123, Italy; Division of Anatomy and Physiopathology, Department of Clinical and Experimental Sciences, University of Brescia, Viale Europa 11, Brescia, 25123, Italy; Department of Civil, Environmental, Architectural Engineering and Mathematics (DICATAM), University of Brescia, Via Valotti 9, Brescia, 25123, Italy; CNR-IMM Bologna, Via Gobetti 101, Bologna, 40129, Italy; PRISM Lab, CNR-INO, Brescia, 25123, Italy; Clinical Proteomics and Metabolomics Unit, Department of Medicine and Surgery, University of Milano-Bicocca, Via Raoul Follereau 3, Vedano al Lambro, 20854, Italy; Department of Applied Chemistry and Chemical Engineering, Faculty of Science, University of Chittagong, Chittagong, 4331, Bangladesh; Laboratory for Stem Cell Manipulation and Cryopreservation, Department of Transfusion Medicine, ASST Spedali Civili, Piazzale Spedali Civili 1, Brescia, 25123, Italy; Orthopedics and Traumatology Unit, ASST Spedali Civili, Via Papa Giovanni XXIII 4, 25063 Gardone Val Trompia, Brescia, 25123, Italy; Department of Information Engineering (DII), University of Brescia, Via Branze 38, Brescia, 25123, Italy
Abstract: Gelatin-dextran hydrogel scaffolds (G-PEG-Dx) were evaluated for their ability to activate the bone marrow human mesenchymal stromal cells (BM-hMSCs) towards mineralization. G-PEG-Dx1 and G-PEG-Dx2, with identical composition but different architecture, were seeded with BM-hMSCs in presence of fetal bovine serum or human platelet lysate (hPL) with or without osteogenic medium. G-PEG-Dx1, characterized by a lower degree of crosslinking and larger pores, was able to induce a better cell colonization than G-PEG-Dx2. At day 28, G-PEG-Dx2, with hPL and osteogenic factors, was more efficient than G-PEG-Dx1 in inducing mineralization. Scanning electron microscopy (SEM) and Raman spectroscopy showed that extracellular matrix produced by BM-hMSCs and calcium-positive mineralization were present along the backbone of the G-PEG-Dx2, even though it was colonized to a lesser degree by hMSCs than G-PEG-Dx1. These findings were con-firmed by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), detecting distinct lipidomic signatures that were associated with the different degree of scaffold mineralization. Our data show that the architecture and morphology of G-PEG-Dx2 is determinant and better than that of G-PEG-Dx1 in promoting a faster mineralization, suggesting a more favora-ble and active role for improving bone repair.
Journal/Review: MATERIALS (BASEL)
Volume: 14 (14) Pages from: 3852-1 to: 3852-23
KeyWords: bone regeneration hydrogel scaffold; mesenchymal stromal cells; human platelet lysate; Raman spectroscopy; MALDI-MSDOI: 10.3390/ma14143852