Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing

Year: 2021

Authors: Rossi F., Giada Magni G., Tatini F., Banchelli M., Cherchi F., Rossi M., Coppi E., Pugliese A.M., Rossi degl’Innocenti D., Alfieri D., Pavone F.S., Pini R., Matteini P.

Autors Affiliation: Istituto di Fisica Applicata “Nello Carrara”, Consiglio Nazionale delle Ricerche (CNR-IFAC), 50019 Florence, Italy; Department of Neuroscience, Psychology, Drug Research and Child Health, Section of Pharmacology and Toxicology, University of Florence, 50139 Florence, Italy; EmoLED s.r.l., Sesto Fiorentino, 50019 Florence, Italy; Department of Physics, University of Florence, 50019 Florence, Italy; European Laboratory for Non-Linear Spectroscopy (LENS), 50019 Florence, Italy; Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche (CNR-INO), 50125 Florence, Italy.

Abstract: In recent years, photobiomodulation (PBM) has been recognized as a physical therapy in wound management. Despite several published research papers, the mechanism underlying photobiomodulation is still not completely understood. The investigation about application of blue light to improve wound healing is a relatively new research area. Tests in selected patients evidenced a stimulation of the healing process in superficial and chronic wounds treated with a blue LED light emitting at 420 nm; a study in animal model pointed out a faster healing process in superficial wound, with an important role of fibroblasts and myofibroblasts. Here, we present a study aiming at evidencing the effects of blue light on the proliferation and metabolism in fibroblasts from healthy skin and keratinocytes. Different light doses (3.43, 6.87, 13.7, 20.6, 30.9 and 41.2 J/cm2) were used to treat the cells, evidencing inhibitory and stimulatory effects following a biphasic dose behavior. Electrophysiology was used to investigate the effects on membrane currents: healthy fibroblasts and keratinocytes showed no significant differences between treated and not treated cells. Raman spectroscopy revealed the mitochondrial Cytochrome C (Cyt C) oxidase dependence on blue light irradiation: a significant decrease in peak intensity of healthy fibroblast was evidenced, while it is less pronounced in keratinocytes. In conclusion, we observed that the blue LED light can be used to modulate metabolism and proliferation of human fibroblasts, and the effects in wound healing are particularly evident when studying the fibroblasts and keratinocytes co-cultures.

Journal/Review: BIOMEDICINES

Volume: 9 (1)      Pages from: 41-1  to: 41-14

More Information: This research was funded by EmoLED s.r.l. The work was partially supported by the Italian Ministry of Foreign Affairs and International Cooperation of Italy through the DESWEAT project (No.PGR01065) to M.B. and P.M.
KeyWords: photobiomodulation, blue light, LED, wound healing
DOI: 10.3390/biomedicines9010041