High-speed multifunctional scanning ion conductance microscopy: Innovative strategies to study dynamic cellular processes
Authors: Tognoni E.
Autors Affiliation: Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche, Via Moruzzi 1, 56124, Pisa, Italy
Abstract: The latest contributions of scanning ion conductance microscopy (SICM) to current research in life sciences are reviewed. The continued efforts toward technical improvements in SICM hardware and software resulted in development of new scanning strategies and instrumental designs. Enhanced highspeed mapping supported the capture of very fast cellular processes at the nanoscale, such as secretory events or cilia movements or the contraction cycle of a cardiomyocyte. The noninvasive scanning capability allowed researchers to monitor the response of individual live cells to chemical stimulations in real time. Access to new kinds of information that can be extracted from experimental data with the support of numerical simulations was demonstrated, thus extending further SICM multifunction capability.
Volume: 28 Pages from: 100738-1 to: 100738-7
KeyWords: Scanning ion conductance microscopy, Nanopipette, Live cell imaging,
Nanoparticles, Nanosensor.DOI: 10.1016/j.coelec.2021.100738Citations: 5data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2023-01-22References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here