Collagen ultrastructural symmetry and its malignant alterations in human breast cancer revealed by polarization-resolved second-harmonic generation microscopy

Year: 2020

Authors: Mercatelli R., Triulzi T., Pavone FS., Orlandi R., Cicchi R.

Autors Affiliation: National Institute of Optics, National Research Council (CNR-INO), Sesto Fiorentino, Italy; Aerospazio Tecnologie s.r.l., Italy; Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy; Department of Physics, University of Florence, Sesto Fiorentino, Italy; European Laboratory for Non-linear Spectroscopy (LENS), Sesto Fiorentino, Italy

Abstract: Several specific alterations of the extracellular matrix can be considered a distinctive hallmark of cancer. In particular, a different morphology of the collagen scaffold is frequently found within the peritumoural environment. In this study, we report about a significant difference in the ultrastructural organization of collagen at the supramolecular
level between the perilesional scaffold and the tumour area in human breast carcinoma samples. In particular, we demonstrated that polarizationresolved second-harmonic generation (P-SHG) microscopy is able to link the altered collagen architecture at the ultrastructural level found in perilesional tissue with a different organization of collagen fibrils at the molecular level.

Journal/Review: JOURNAL OF BIOPHOTONICS

Volume: 13 (8)      Pages from: e202000159  to: e202000159

More Information: Horizon 2020 Framework Programme, Grant/Award Number: 654148 & 732111; Ministero della Salute, Grant/Award Number: GR-2011-02349626; Seventh Framework Programme, Grant/Award Number: EU FP7 BiophotonicsPlus – LITE; Associazione Italiana per la Ricerca sul Cancro, Grant/Award Number: 12162; Horizon 2020; Ministry of Health; Tuscany Region
KeyWords: breast cancer, collagen, polarization, SHG microscopy
DOI: 10.1002/jbio.202000159

Citations: 21
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