Scientific Results

Collagen ultrastructural symmetry and its malignant alterations in human breast cancer revealed by Polarization SHG microscopy

Year: 2020

Authors: Mercatelli R., Triulzi T., Pavone FS., Orlandi R., Cicchi R.

Autors Affiliation: 1National Institute of Optics, National
Research Council (CNR-INO), Sesto
Fiorentino, Italy
2Aerospazio Tecnologie s.r.l., Italy
3Fondazione IRCCS Istituto Nazionale dei
Tumori, Milan, Italy
4Department of Physics, University of
Florence, Sesto Fiorentino, Italy
5European Laboratory for Non-linear
Spectroscopy (LENS), Sesto Fiorentino, Italy

Abstract: Several specific alterations of
the extracellular matrix can be
considered a distinctive hallmark
of cancer. In particular, a
different morphology of the
collagen scaffold is frequently
found within the peritumoural environment. In this study, we report about a
significant difference in the ultrastructural organization of collagen at the supramolecular
level between the perilesional scaffold and the tumour area in human
breast carcinoma samples. In particular, we demonstrated that polarizationresolved
second-harmonic generation (P-SHG) microscopy is able to link the
altered collagen architecture at the ultrastructural level found in perilesional tissue
with a different organization of collagen fibrils at the molecular level.


Volume: 13      Pages from: e202000159  to: e202000159

KeyWords: breast cancer, collagen, polarization, SHG microscopy
DOI: 10.1002/jbio.202000159