Simultaneous fluorescence lifetime and Raman fiber-based mapping of tissues
Authors: Lagarto JL., Shcheslavskiy V., Pavone FS., Cicchi R.
Autors Affiliation: National Institute of Optics, National Research Council (INO-CNR), Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy;
European Laboratory for Non-linear Spectroscopy (LENS), Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy; Becker & Hickl GmbH, Nunsdorfer Ring 7-9, 12277 Berlin, Germany: Privolzhskiy Medical Research University, 603005 Nizhny Novgorod, Russia; Department of Physics, University of Florence, Via G. Sansone 1, 50019, Sesto Fiorentino, Italy
Abstract: We report the development of a novel, to the best of our knowledge, fiber-based system to realize coregistered simultaneous acquisition of fluorescence lifetime (FL) data and Raman spectra from the same area. FL measurements by means of time-correlated single photon counting are realized with periodic out-of-phase external illumination of the field of view, enabling acquisition of data under bright illumination of the specimen. Raman measurements in the near-infrared are realized asynchronously. We present a detailed characterization of this technique and validate its potential to report intrinsic contrast. Fiber-based FL and Raman maps report complementary structural, compositional, and molecular contrast in biological tissues with diverse compositional features.
Journal/Review: OPTICS LETTERS
Volume: 45 (8) Pages from: 2247 to: 2250