Scientific Results

Simultaneous fluorescence lifetime and Raman fiber-based mapping of tissues

Year: 2020

Authors: Lagarto JL., Shcheslavskiy V., Pavone FS., Cicchi R.

Autors Affiliation: 1National Institute of Optics, National Research Council (INO-CNR), Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy
2European Laboratory for Non-linear Spectroscopy (LENS), Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy
3Becker & Hickl GmbH, Nunsdorfer Ring 7-9, 12277 Berlin, Germany
4Privolzhskiy Medical Research University, 603005 Nizhny Novgorod, Russia
5Department of Physics, University of Florence, Via G. Sansone 1, 50019, Sesto Fiorentino, Italy

Abstract: We report the development of a novel, to the best of our
knowledge, fiber-based system to realize coregistered simultaneous
acquisition of fluorescence lifetime (FL) data and
Raman spectra from the same area. FL measurements by
means of time-correlated single photon counting are realized
with periodic out-of-phase external illumination of
the field of view, enabling acquisition of data under bright
illumination of the specimen. Raman measurements in the
near-infrared are realized asynchronously. We present a
detailed characterization of this technique and validate its
potential to report intrinsic contrast. Fiber-based FL and
Raman maps report complementary structural, compositional,
and molecular contrast in biological tissues with
diverse compositional features.

Journal/Review: OPTICS LETTERS

Volume: 45      Pages from: 2247  to: 2250

KeyWords: Raman spectroscopy, lifetime spectroscopy, real-time imaging
DOI: 10.1364/OL.389300

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