Revealing membrane alteration in cellsoverexpressing CA IX and EGFR by Surface-Enhanced Raman Scattering
Authors: Rusciano G., Sasso E., Capaccio A., Zambrano N., Sasso A.
Autors Affiliation: Univ Naples Federico II, Dept Phys E Pancini, Complesso Univ Monte S Angelo,Via Cintia, I-80126 Naples, Italy; Natl Res Council CNR, Natl Inst Opt INO, Via Campi Flegrei 34, I-80078 Pozzuoli, NA, Italy; Univ Naples Federico II, Dept Mol Med & Med Biotechnol, Via S Pansini 5, I-80131 Naples, Italy; CEINGE Adv Biotechnol SCaRL, Via G Salvatore 486, I-80145 Naples, Italy; Nouscom SRL, Rome, Italy
Abstract: Sensitive detection of altered proteins expression in plasma membranes is of fundamental importance, for both diagnostic and prognostic purposes. Surface-Enhanced Raman Scattering (SERS) has proven to be a quite sensitive approach to detect proteins, even in very diluted samples. However, proteins detection in complex environment, such as the cellular membrane, is still a challenge. Herein, we demonstrate a SERS-based platform to reveal the overexpression of target proteins in cell membranes. As a proof of concept, we implemented ectopic expression of carbonic anhydrase IX (CA IX) and epidermal growth factor receptor (EGFR) in the plasma membrane of the SKOV3 tumor cell line. Our outcomes demonstrate that SERS signals from cells put in contact with a hyperuniform SERS substrate allow highlighting subtle differences in the biochemical composition of cell membranes, normally hidden in spontaneous Raman confocal microscopy. This opens new opportunities for a label-free membrane analysis and bio-sensing in a broader sense.
Journal/Review: SCIENTIFIC REPORTS
Volume: 9 Pages from: 1832-1 to: 1832-10
KeyWords: CARBONIC-ANHYDRASE IX; SPECTROSCOPY; PROTEINDOI: 10.1038/s41598-018-37997-3Citations: 2data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2020-10-18References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here