Wide-field imaging of cortical neuronal activity with red-shifted functional indicators during motor task execution
Authors: Montagni E., Resta F., Conti E., Scaglione A., Pasquini M., Micera S., Allegra Mascaro, AL., Pavone FS.
Autors Affiliation: Univ Florence, European Lab Nonlinear Spect, Florence, Italy; Scuola Super Sant Anna, Pisa, Italy; Ecole Polytech Fed Lausanne, Lausanne, Switzerland; CNR, Neurosci Inst, Pisa, Italy; CNR, Natl Inst Opt, Sesto Fiorentino, Italy; Univ Florence, Dept Phys & Astron, Sesto Fiorentino, Italy
Abstract: Genetically encoded calcium indicators (GECIs) can be used to monitor the intracellular concentration of free calcium in populations of neurons. GECIs with long emission wavelengths are particularly attractive for deep tissue microscopy in vivo, and have the additional advantage of avoiding spectral overlap with commonly used neuronal actuators such as channelrhodopsin. The aim of this work is to evaluate the performances of four red-shifted GECIs (jRCaMP1a, jRCaMP1b, jRGECO1a, jRGECO1b) using both ex vivo and in vivo experimental techniques. Cortical neurons were infected with adeno-associated virus (AAV) expressing one of the red GECI variants. First we characterized the transfection ex vivo in terms of extension and intensity of the indicator. Next, we monitored the neuronal activity over the right cortical hemisphere of a jRCaMP1a-transfected mouse during a goal-directed motor task. To this aim, we combined wide-field fluorescence microscopy with a robotic device for simultaneous recording of cortical neuronal activity, force applied and forelimb position during task execution. Our results show that jRCaMP1a has sufficient sensitivity to monitor in vivo neuronal activity simultaneously over multiple functional areas, and can be successfully used to perform longitudinal imaging sessions in awake mice.
Journal/Review: JOURNAL OF PHYSICS D-APPLIED PHYSICS
Volume: 52 (7) Pages from: 074001-1 to: 074001-10
KeyWords: wide-field microscopy; GECI; calcium imaging; fluorescence; in vivoDOI: 10.1088/1361-6463/aaf26cCitations: 3data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2020-08-02References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here