Morpho-mechanics of human collagen superstructures revealed by all-optical correlative micro-spectroscopies
Authors: Mercatelli R., Mattana S., Capozzoli L., Ratto F., Rossi F., Pini R., Fioretto D., Pavone F.S., Caponi S., Cicchi R.
Autors Affiliation: National Institute of Optics, National Research Council (CNR-INO), Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy; Department of Physics and Geology, University of Perugia, Via Alessandro Pascoli, I-06123 Perugia, Italy.
Institute of Applied Physics “Nello Carrara”, National Research Council (CNRIFAC), Via Madonna del Piano 10, I-50019 Sesto Fiorentino, Italy; Center of Electron Microscopy “Laura Bonzi” (Ce.M.E), Institute of Chemistry of Organometallic Compounds, National Research Council (CNR-ICCOM), Via Madonna del Piano 10, I-50019 Sesto Fiorentino, Italy.
CEMIN-Center of Excellence for Innovative Nanostructured Material, Via Alessandro Pascoli, I-06123 Perugia, Italy.
European Laboratory for Non-linear Spectroscopy (LENS), Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy.
Department of Physics, University of Florence, Via Giovanni Sansone 1, I-50019 Sesto Fiorentino, Italy; Institute of Materials, National Research Council (CNR-IOM), Unit of Perugia, c/o Department of Physics and Geology, University of Perugia, Via A. Pascoli, I-06123 Perugia, Italy
Abstract: In every biological tissue, morphological and topological properties strongly affect its mechanical features and behaviour, so that ultrastructure, composition and mechanical parameters are intimately connected. Overall, it is their correct interplay that guarantees the tissue functionality. The development of experimental methods able to correlate these properties would open new opportunities both in the biological and the biomedical fields. Here, we report a correlative study intended to map supramolecular morphology, biochemical composition and viscoelastic parameters of collagen by all-optical microscopies. In particular, using human corneal tissue as a benchmark, we correlate Second-Harmonic Generation maps with mechanical and biochemical imaging obtained by Brillouin and Raman microspectroscopy. The study highlights how subtle variations in supramolecular organization originate the peculiar mechanical behavior of different subtypes of corneal lamellae. The presented methodology paves the way to the non-invasive assessment of tissue morphomechanics in biological as well as synthetic materials.
Journal/Review: COMMUNICATIONS BIOLOGY
Volume: 2 Pages from: 117-1 to: 117-10
KeyWords: collagen, SHG microscopy, Brillouin light scattering, Raman microscopyDOI: 10.1038/s42003-019-0357-yCitations: 23data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2022-01-23References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here