Bimodal spectroscopy of formalin fixed samples to discriminate dysplastic and tumor brain tissues
Authors: Anand S., Cicchi R., Giordano F., Buccoliero A.M., Guerrini R., Pavone F.S.
Autors Affiliation: European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Via Nello Carrara, 1, Sesto Fiorentino, 50019, Italy; National Institute of Optics, National Research Council (INO-CNR), Largo Enrico Fermi 6, Florence, 50125, Italy; Division of Neurosurgery, Department of Neuroscience i, Anna Meyer Pediatric Hospital, Viale Gaetano Pieraccini 24, Florence, 50141, Italy; Division of Pathology, Department of Critical Care Medicine and Surgery, University of Florence, Viale Giovanni Battista Morgagni 85, Florence, 50134, Italy; Department of Physics, University of Florence, Via Giovanni Sansone 1, Sesto Fiorentino, 50019, Italy
Abstract: Biomedical spectroscopy has gained attention in the past few years for disease diagnosis. Fluorescence and Raman spectroscopies provide finger-print information related to biochemical and morphological alterations when tissues progress from the normal to a malignant stage. Usually, freshly excised tissue specimens are preferred for bio-spectroscopic studies. However, ethical issues, sample availability and distance between the surgery room and the laboratory provide an impelling restriction for in-vitro spectroscopic studies using freshly excised samples. After surgical resection tissues are fixed in 4% formalin for histological studies under a light microscope. The process of fixation prevents degradation of tissues. In this study, we probe the use of formalin fixed sample for differentiating normal and dysplastic brain tissues using fluorescence and Raman spectroscopies. It was found that fluorescence spectral profile changes in the wavelength range from 550-750 nm between dysplastic and tumor samples. Also, significant differences were found in the Raman spectral profiles of such samples. The results indicate a potential diagnostic application of spectroscopy in formalin fixed brain samples for differentiating dysplastic and tumor brain tissues.
Journal/Review: LATVIAN JOURNAL OF PHYSICS AND TECHNICAL SCIENCES
Volume: 51 (5) Pages from: 14 to: 20
More Information: The authors acknowledge the funding from Fondazione Pisa in framework of the project “Diagnostic technology for the post-operative monitoring of pediatric brain tumors”, European Union Seventh Framework Programme (FP7/2007-2013) under grant agreements number 228334 and 284464, and Italian Ministry for Education, University and Research in the framework of the Flagship Project NANOMAX. Financial support by “Ente Cassa di Risparmio di Firenze” (Private Foundation) is acknowledged.KeyWords: Brain tumor; Dysplasia; Fluorescence; Formalin fixation; Raman spectroscopyDOI: 10.2478/lpts-2014-0027Citations: 1data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2020-01-19References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here